PROJECT II - IGM-BINDING EPITOPES IN INJURED TISSUE

项目 II - 受损组织中的 IGM 结合表位

• 批准号: 6674470
• 负责人: FRANCIS D MOORE
• 金额: $ 16.5万
• 依托单位: BRIGHAM AND WOMEN'S HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-04-01 至 2006-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6674470
• 关键词: B lymphocyte; antibody; antigens; cell surface receptors; clinical research; complement; enzyme linked immunosorbent assay; flow cytometry; histochemistry /cytochemistry; human subject; hybridomas; immunoglobulin M; injury; leukocyte activation /transformation; protein binding; reperfusion; tissue /cell culture; western blottings

Project II seeks to develop the finding that a single clone of murine IgM is pathogenic for intestinal reperfusion injury in antibody-deficient animals. The first aim will be to determine whether this antibody binds to definable antigens in murine tissue, whether injured or not. This investigation may lead to discovery of the identity of the murine antigen. In parallel, studies of human peritoneal B lymphocytes will be initiated in an attempt to determine whether the products of these cells have activity which is similar to the activity of the monoclonal IgM. Finally, support with histochemistry and complement assays will be given to other Projects within the Trauma Center.

项目II旨在发现小鼠IgM的单个克隆对肠道疾病具有致病性， 再灌注损伤的抗体缺陷的动物。第一个目标将是确定这是否 抗体与鼠组织中可确定的抗原结合，无论是否受损。这次调查 可能导致发现鼠抗原的身份。与此同时，将启动对人腹膜B淋巴细胞的研究，试图确定这些细胞的产物是否具有与单克隆IgM活性相似的活性。最后，组织化学和补体分析的支持将给予创伤中心内的其他项目。