Genomic Basis of Vertebrate Diversity

脊椎动物多样性的基因组基础

• 批准号: 6626049
• 负责人: WILLIAM S TALBOT
• 金额: $ 299.24万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-04-19 至 2007-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6626049
• 关键词: Chordata; biochemical evolution; developmental genetics; functional /structural genomics; gene duplication; gene expression; genetic polymorphism; in situ hybridization; informatics; linkage mapping; molecular cloning; quantitative trait loci; restriction fragment length polymorphism; species difference; zebrafish

DESCRIPTION (provided by applicant): This CEGS will use an innovative combination of approaches to address two fundamental questions in genome biology: What do our genes do, and where did we come from? Rapid progress in genomics has provided nearly complete sequences for several organisms. Comparative analysis suggests many fundamental pathways and gene networks are conserved between organisms. And yet, the morphology, behavior, physiology, and disease susceptibility of different species are obviously and profoundly different. What are the mechanisms that generate new functions for genes, new physiological traits, and the unique form and functions of different species? Has the great variety of life forms been created by changes in gene number, by alterations in the functional attributes of particular proteins, or by diversification of the regulatory mechanisms that control where and when genes are expressed? This CEGS proposes a pioneering analysis of vertebrate diversity using a combination of techniques from structural and functional genomic and traditional genetics in zebrafish and sticklebacks. The unique experimental advantages of these two models will make it possible to take complementary approaches. The "bottom-up" approach will test the diversification in expression and genetic function of duplicated gene pairs, a major hallmark of the vertebrate genome. In situ hybridization analysis will be used to compare the expression patterns of 2500 genes. Morpholino knockout experiments will test how the functions of duplicated genes diverge, and generate a database of gene functions for many genes identified in sequencing projects. The complementary "top-down" approach will begin with naturally occurring species of sticklebacks that show profound differences in size, anatomy, and physiological traits. Genetic crosses will be used to identify the number and location of genetic changes that create the anatomical and physiological differences between recently evolved species from different regions around the world. Development of genetic and physical mapping resources for sticklebacks will make it possible to identify the actual genes and mutations responsible for evolutionary change. Immediate data release, free access to reagents, and an annual summer training course in fish genomic and genetics will ensure that the innovative approaches and results from this research will be widely disseminated to the research community. This unique combination of approaches will establish a completely new and detailed understanding of the genomic mechanisms responsible for morphological and physiological differences between living forms.

描述（由申请人提供）：本CEGS将使用创新的 解决基因组中两个基本问题的方法组合 生物学：我们的基因是做什么的，我们从哪里来？迅速进展 基因组学已经提供了几种生物体的几乎完整的序列。 比较分析表明，许多基本的途径和基因网络， 在生物体之间保存。然而，形态学，行为，生理学， 不同物种的感病性差异显著 不同.基因产生新功能的机制是什么？ 生理特征，以及不同物种的独特形式和功能？ 生命形式的多样性是由基因数量的变化， 特定蛋白质的功能属性的改变，或者 控制基因在何时何地的调节机制的多样化 是表达？这CEGS提出了一个开创性的分析脊椎动物的多样性 使用结构和功能基因组技术的组合， 斑马鱼和刺鱼的传统遗传学。独特的实验 这两种模式的优势将使我们有可能采取互补的方式。 接近。“自下而上”的方法将检验 重复的基因对的表达和遗传功能，这是 脊椎动物的基因组原位杂交分析将用于比较 2500个基因的表达模式。吗啉基敲除实验将 测试重复基因的功能是如何分化的，并生成一个数据库， 在测序项目中鉴定的许多基因的基因功能。的 一种互补的“自上而下”的方法将开始于自然发生的物种 它们在大小、解剖结构和 生理特征遗传杂交将用于确定数量和 基因变化的位置，这些变化创造了解剖学和生理学上的 来自不同地区的最近进化的物种之间的差异 世界刺鱼遗传和物理图谱资源的开发 将使我们有可能确定真正的基因和突变 for evolutionary进化change更改.立即发布数据，免费获取试剂，以及 鱼类基因组和遗传学方面的年度夏季培训班将确保， 这项研究的创新方法和成果将广泛应用于 传播到研究界。这种独特的方法组合 将建立一个全新的和详细的了解基因组 负责形态和生理差异的机制 生命形式。