Molecular Basis of Pathogen-Induced Cell Death in Plants
病原体诱导植物细胞死亡的分子基础
基本信息
- 批准号:6831380
- 负责人:
- 金额:$ 2.7万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:1996
- 资助国家:美国
- 起止时间:1996-05-01 至 2005-06-30
- 项目状态:已结题
- 来源:
- 关键词:Arabidopsisankyrinsapoptosisbiological signal transductioncell typechlorophyllchloroplastsgene expressionimmunoelectron microscopylight microscopymembrane proteinsmitochondriamolecular cloningmolecular geneticspathologic processplant diseasesplant geneticsplant growth regulatorsprotein protein interactionregulatory genesingle cell analysisvideo microscopyyeast two hybrid system
项目摘要
This competing renewal outlines experiments that combine the powerful molecular genetics of the model plant Arabidopsis with cell biological approaches to examine the function of several key genes involved in pathogenesis-associated programmed cell death (PCD) regulation and execution. Understanding the control of PCD is important for developing strategies to manipulate PCD to prevent or cure diseases involving excess or insufficient PCD induction. Known in plants as the "hypersensitive response" (HR), PCD aids in limiting the spread of some pathogens and/or amplifying other defenses. Plants have a second, slower PCD response, called susceptible cell death, that facilitates pathogen growth and transmission and is triggered by pathogens that do not cause an HR. Both types of PCD are subject to genetic control and in some cases both require a common signaling molecule called salicylic acid (SA). However, initiation of the HR in response to some pathogens does not require SA. These observations have raised important questions as to whether the regulation and/or execution of PCD occurs by multiple independent mechanisms or whether there are some core steps. To address these questions and to elucidate the steps required for the control of PCD associated with the HR and susceptible cell death, several genes termed ACCELERATED CELL DEATH (ACD) important for cell death and defense regulation were identified. The proposed research addresses the mechanism of action of a novel SA-dependent signaling component of the cell death and disease resistance pathway called ACD6, the characterization of the SA-dependent ACD5 gene important for cell death during disease and the determination an SA-independent mechanism whereby chlorophyll catabolites, structurally related to the PCD-inducing protoporphyrin IX in animals, induce cell death during disease. A more open ended aspect of the research program is the identification of additional genes, through the employment of genetic screens, important for the positive and negative regulation of cell death that occurs during disease and the HR. Finally, an important goal is to exploit the acd and other mutants in the development of cell biological assays to directly visualize and study the properties and signaling requirements of single dying cells and to determine the involvement of mitochondria and/or chloroplasts in coordinating PCD.
这种竞争性的更新概述了联合收割机结合模式植物拟南芥的强大的分子遗传学与细胞生物学方法,以检查参与病程相关的程序性细胞死亡(PCD)的调节和执行的几个关键基因的功能的实验。 了解PCD的控制对于开发策略来操纵PCD以预防或治疗涉及PCD诱导过量或不足的疾病是重要的。 在植物中称为“过敏反应”(HR),PCD有助于限制某些病原体的传播和/或放大其他防御。 植物有第二个,较慢的PCD反应,称为易感细胞死亡,这有利于病原体的生长和传播,并由病原体触发,不会导致HR. Both类型的PCD受到遗传控制,在某些情况下,都需要一个共同的信号分子称为水杨酸(SA)。 然而,针对某些病原体启动HR不需要SA。 这些观察结果提出了重要的问题,即PCD的监管和/或执行是否由多个独立的机制进行,或者是否有一些核心步骤。 为了解决这些问题并阐明与HR和易感细胞死亡相关的PCD控制所需的步骤,鉴定了几个对细胞死亡和防御调节重要的称为加速细胞死亡(ACD)的基因。 拟议的研究解决了一种新的SA依赖的细胞死亡和抗病途径的信号成分ACD 6的作用机制,SA依赖的ACD 5基因的表征对疾病期间的细胞死亡很重要,并确定了一种SA独立的机制,即叶绿素催化剂,结构上与PCD诱导的动物原卟啉IX相关,在疾病期间诱导细胞死亡。 该研究计划的一个更开放的方面是通过采用遗传筛选来鉴定其他基因,这些基因对疾病和HR期间发生的细胞死亡的正向和负向调节很重要。一个重要的目标是在细胞生物学测定的发展中利用ACD和其它突变体并确定线粒体和/或叶绿体参与协调PCD。
项目成果
期刊论文数量(0)
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科研奖励数量(0)
会议论文数量(0)
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Jean T. Greenberg其他文献
Protocol for analyzing the movement and uptake of isotopically labeled signaling molecule azelaic acid in Arabidopsis
分析拟南芥中同位素标记信号分子壬二酸的运动和摄取的方案
- DOI:
- 发表时间:
2024 - 期刊:
- 影响因子:0
- 作者:
Suruchi Roychoudhry;Jean T. Greenberg;Nicolás M Cecchini - 通讯作者:
Nicolás M Cecchini
Jean T. Greenberg的其他文献
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{{ truncateString('Jean T. Greenberg', 18)}}的其他基金
Exploiting pathogen-induced cell death to create disease resistant plants:R01GM05
利用病原体诱导的细胞死亡来创造抗病植物:R01GM05
- 批准号:
7791369 - 财政年份:2008
- 资助金额:
$ 2.7万 - 项目类别:
Exploiting pathogen-induced cell death to create disease resistant plants:R01GM05
利用病原体诱导的细胞死亡来创造抗病植物:R01GM05
- 批准号:
7429160 - 财政年份:2008
- 资助金额:
$ 2.7万 - 项目类别:
Exploiting pathogen-induced cell death to create disease resistant plants:R01GM05
利用病原体诱导的细胞死亡来创造抗病植物:R01GM05
- 批准号:
7595925 - 财政年份:2008
- 资助金额:
$ 2.7万 - 项目类别:
Molecular Basis of Pathogen-Induced Cell Death in Plants
病原体诱导植物细胞死亡的分子基础
- 批准号:
7488630 - 财政年份:1996
- 资助金额:
$ 2.7万 - 项目类别:
Molecular Basis of Pathogen-Induced Cell Death in Plants
病原体诱导植物细胞死亡的分子基础
- 批准号:
6608802 - 财政年份:1996
- 资助金额:
$ 2.7万 - 项目类别:
Molecular Basis of Pathogen-Induced Cell Death in Plants
病原体诱导植物细胞死亡的分子基础
- 批准号:
7599789 - 财政年份:1996
- 资助金额:
$ 2.7万 - 项目类别:
Molecular Basis of Pathogen-Induced Cell Death in Plants
病原体诱导植物细胞死亡的分子基础
- 批准号:
6399224 - 财政年份:1996
- 资助金额:
$ 2.7万 - 项目类别:
Molecular Basis of Pathogen-Induced Cell Death in Plants
病原体诱导植物细胞死亡的分子基础
- 批准号:
7293589 - 财政年份:1996
- 资助金额:
$ 2.7万 - 项目类别:
Molecular Basis of Pathogen-Induced Cell Death in Plants
病原体诱导植物细胞死亡的分子基础
- 批准号:
7194497 - 财政年份:1996
- 资助金额:
$ 2.7万 - 项目类别:
Molecular Basis of Pathogen-Induced Cell Death in Plants
病原体诱导植物细胞死亡的分子基础
- 批准号:
6519750 - 财政年份:1996
- 资助金额:
$ 2.7万 - 项目类别:
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