Molecular Basis of Pathogen-Induced Cell Death in Plants

病原体诱导植物细胞死亡的分子基础

基本信息

  • 批准号:
    7293589
  • 负责人:
  • 金额:
    $ 33.54万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    1996
  • 资助国家:
    美国
  • 起止时间:
    1996-05-01 至 2010-08-31
  • 项目状态:
    已结题

项目摘要

DESCRIPTION (provided by applicant): Experiments in this competing renewal combine the powerful genetic tools of the model plant Arabidopsis with cell biological and biochemical approaches to examine a major signaling pathway that controls innate immunity and programmed cell death (PCD). The control of plant defenses has many parallels with human innate immunity. Furthermore, PCD has similar features and regulation in plants and humans. Our long term goal is to understand the molecular basis of PCD regulation and execution. This is essential for developing strategies to manipulate PCD to prevent or cure diseases involving excess or insufficient PCD induction in both plants and humans. What is learned from studying plant immunity and PCD will be a paradigm for understanding similar events in humans. Experiments described herein build on previous work on two Arabidopsis genes, ACD6 and ACD2. ACD6 is a novel integral plasma membrane protein with an N- terminal ankyrin repeat domain that controls defense and PCD in plants. Ankyrin repeats are involved in protein-protein interactions in plants, humans and many other organisms. ACD6 is important for both local and systemic signaling during infection. ACD2 is a novel protein that controls the activation and extent of PCD during infection by controlling the levels or reactivity of an endogenous PCD-inducing molecule that is either a porphyrin or a porphyrin-like molecule. Thus, infection activates ACD6 to regulate defenses and PCD and ACD2 modulates the timing and extent of PCD to prevent excess tissue damage. The proposed research aims to: (i) determine the mechanism of action of ACD6 in activating PCD and disease resistance using molecular genetic and biochemical approaches; (2) determine the mechanism of action of ACD2 by combining molecular genetic, biochemical and physiological experiments; and (3) discern the properties and signaling requirements of cells that die due to porphyrin treatment (a surrogate for infection) using cell biological approaches. Porphyrins are important in anti-tumor therapies being developed. Therefore, it is important to know as much as possible about the effects that porphyrins have on cells. Dis-regulation of porphyrins in human (a condition called porphyria) causes severe disease to humans. ACD2 may provide a way to help people with this disease, since it likely has the potential to detoxify porphyrins. A number of human diseases are also caused by the malfunction of ankyrin-containing proteins. This work will generate important information about the whole class of ankyrin proteins that can be applied to understanding and possibly interrupting some human diseases. These studies will unravel common processes in plants and humans that can be manipulated to treat diseases caused by too much or too little cell damage. Because experimental progress using a plant model is rapid, the results obtained and quickly be related to human biology and disease.
描述(申请人提供):在这个竞争性更新的实验中,将模式植物拟南芥强大的遗传工具与细胞生物学和生化方法相结合,以检查控制先天免疫和程序性细胞死亡(PCD)的主要信号通路。植物防御的控制与人类的先天免疫有许多相似之处。此外,PCD在植物和人类中也有类似的特征和调节。我们的长期目标是了解PCD调控和执行的分子基础。这对于制定控制PCD的策略以预防或治愈涉及植物和人类PCD诱导过多或不足的疾病至关重要。从研究植物免疫和PCD中学到的东西将成为理解人类类似事件的范例。这里描述的实验建立在先前对两个拟南芥基因ACD6和ACD2进行的工作的基础上。ACD6是一种新的完整的质膜蛋白,含有N-末端的锚蛋白重复结构域,控制植物的防御和PCD。在植物、人类和许多其他生物体中,锚蛋白重复序列参与蛋白质之间的相互作用。在感染过程中,ACD6对局部和系统信号都很重要。ACD2是一种新的蛋白质,它通过控制内源性PCD诱导分子的水平或反应性来控制PCD在感染过程中的激活和程度,内源性PCD诱导分子是一种卟啉或类似卟啉的分子。因此,感染激活ACD6来调节防御,而PCD和ACD2调节PCD的时间和程度,以防止过度的组织损伤。这项研究的目的是:(1)利用分子遗传学和生物化学方法确定ACD6激活PCD和抗病的作用机制;(2)结合分子遗传学、生化和生理学实验确定ACD2的作用机制;(3)利用细胞生物学方法识别因卟啉处理(感染的替代品)而死亡的细胞的特性和信号需求。 卟啉在正在开发的抗肿瘤疗法中非常重要。因此,尽可能多地了解卟啉对细胞的影响是很重要的。卟啉在人体内的失调(一种称为卟啉症的情况)会给人类带来严重的疾病。ACD2可能提供了一种帮助患有这种疾病的人的方法,因为它很可能具有为卟啉解毒的潜力。许多人类疾病也是由含有锚蛋白的蛋白质故障引起的。这项工作将产生有关整个类别的锚定蛋白的重要信息,这些信息可用于理解并可能阻断某些人类疾病。这些研究将揭开植物和人类的常见过程,这些过程可以被操纵来治疗由细胞损伤过多或过少引起的疾病。由于使用植物模型的实验进展迅速,所获得的结果很快就与人类生物学和疾病有关。

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

Jean T. Greenberg其他文献

Protocol for analyzing the movement and uptake of isotopically labeled signaling molecule azelaic acid in Arabidopsis
分析拟南芥中同位素标记信号分子壬二酸的运动和摄取的方案
  • DOI:
  • 发表时间:
    2024
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Suruchi Roychoudhry;Jean T. Greenberg;Nicolás M Cecchini
  • 通讯作者:
    Nicolás M Cecchini

Jean T. Greenberg的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('Jean T. Greenberg', 18)}}的其他基金

Exploiting pathogen-induced cell death to create disease resistant plants:R01GM05
利用病原体诱导的细胞死亡来创造抗病植物:R01GM05
  • 批准号:
    7429160
  • 财政年份:
    2008
  • 资助金额:
    $ 33.54万
  • 项目类别:
Exploiting pathogen-induced cell death to create disease resistant plants:R01GM05
利用病原体诱导的细胞死亡来创造抗病植物:R01GM05
  • 批准号:
    7791369
  • 财政年份:
    2008
  • 资助金额:
    $ 33.54万
  • 项目类别:
Exploiting pathogen-induced cell death to create disease resistant plants:R01GM05
利用病原体诱导的细胞死亡来创造抗病植物:R01GM05
  • 批准号:
    7595925
  • 财政年份:
    2008
  • 资助金额:
    $ 33.54万
  • 项目类别:
Molecular Basis of Pathogen-Induced Cell Death in Plants
病原体诱导植物细胞死亡的分子基础
  • 批准号:
    7488630
  • 财政年份:
    1996
  • 资助金额:
    $ 33.54万
  • 项目类别:
Molecular Basis of Pathogen-Induced Cell Death in Plants
病原体诱导植物细胞死亡的分子基础
  • 批准号:
    6608802
  • 财政年份:
    1996
  • 资助金额:
    $ 33.54万
  • 项目类别:
Molecular Basis of Pathogen-Induced Cell Death in Plants
病原体诱导植物细胞死亡的分子基础
  • 批准号:
    7599789
  • 财政年份:
    1996
  • 资助金额:
    $ 33.54万
  • 项目类别:
Molecular Basis of Pathogen-Induced Cell Death in Plants
病原体诱导植物细胞死亡的分子基础
  • 批准号:
    6399224
  • 财政年份:
    1996
  • 资助金额:
    $ 33.54万
  • 项目类别:
Molecular Basis of Pathogen-Induced Cell Death in Plants
病原体诱导植物细胞死亡的分子基础
  • 批准号:
    6831380
  • 财政年份:
    1996
  • 资助金额:
    $ 33.54万
  • 项目类别:
Molecular Basis of Pathogen-Induced Cell Death in Plants
病原体诱导植物细胞死亡的分子基础
  • 批准号:
    7194497
  • 财政年份:
    1996
  • 资助金额:
    $ 33.54万
  • 项目类别:
MOLECULAR BASIS OF PATHOGEN INDUCED CELL DEATH IN PLANTS
病原体诱导植物细胞死亡的分子基础
  • 批准号:
    6180898
  • 财政年份:
    1996
  • 资助金额:
    $ 33.54万
  • 项目类别:

相似海外基金

Rational design of rapidly translatable, highly antigenic and novel recombinant immunogens to address deficiencies of current snakebite treatments
合理设计可快速翻译、高抗原性和新型重组免疫原,以解决当前蛇咬伤治疗的缺陷
  • 批准号:
    MR/S03398X/2
  • 财政年份:
    2024
  • 资助金额:
    $ 33.54万
  • 项目类别:
    Fellowship
Re-thinking drug nanocrystals as highly loaded vectors to address key unmet therapeutic challenges
重新思考药物纳米晶体作为高负载载体以解决关键的未满足的治疗挑战
  • 批准号:
    EP/Y001486/1
  • 财政年份:
    2024
  • 资助金额:
    $ 33.54万
  • 项目类别:
    Research Grant
CAREER: FEAST (Food Ecosystems And circularity for Sustainable Transformation) framework to address Hidden Hunger
职业:FEAST(食品生态系统和可持续转型循环)框架解决隐性饥饿
  • 批准号:
    2338423
  • 财政年份:
    2024
  • 资助金额:
    $ 33.54万
  • 项目类别:
    Continuing Grant
Metrology to address ion suppression in multimodal mass spectrometry imaging with application in oncology
计量学解决多模态质谱成像中的离子抑制问题及其在肿瘤学中的应用
  • 批准号:
    MR/X03657X/1
  • 财政年份:
    2024
  • 资助金额:
    $ 33.54万
  • 项目类别:
    Fellowship
CRII: SHF: A Novel Address Translation Architecture for Virtualized Clouds
CRII:SHF:一种用于虚拟化云的新型地址转换架构
  • 批准号:
    2348066
  • 财政年份:
    2024
  • 资助金额:
    $ 33.54万
  • 项目类别:
    Standard Grant
The Abundance Project: Enhancing Cultural & Green Inclusion in Social Prescribing in Southwest London to Address Ethnic Inequalities in Mental Health
丰富项目:增强文化
  • 批准号:
    AH/Z505481/1
  • 财政年份:
    2024
  • 资助金额:
    $ 33.54万
  • 项目类别:
    Research Grant
ERAMET - Ecosystem for rapid adoption of modelling and simulation METhods to address regulatory needs in the development of orphan and paediatric medicines
ERAMET - 快速采用建模和模拟方法的生态系统,以满足孤儿药和儿科药物开发中的监管需求
  • 批准号:
    10107647
  • 财政年份:
    2024
  • 资助金额:
    $ 33.54万
  • 项目类别:
    EU-Funded
BIORETS: Convergence Research Experiences for Teachers in Synthetic and Systems Biology to Address Challenges in Food, Health, Energy, and Environment
BIORETS:合成和系统生物学教师的融合研究经验,以应对食品、健康、能源和环境方面的挑战
  • 批准号:
    2341402
  • 财政年份:
    2024
  • 资助金额:
    $ 33.54万
  • 项目类别:
    Standard Grant
Ecosystem for rapid adoption of modelling and simulation METhods to address regulatory needs in the development of orphan and paediatric medicines
快速采用建模和模拟方法的生态系统,以满足孤儿药和儿科药物开发中的监管需求
  • 批准号:
    10106221
  • 财政年份:
    2024
  • 资助金额:
    $ 33.54万
  • 项目类别:
    EU-Funded
Recite: Building Research by Communities to Address Inequities through Expression
背诵:社区开展研究,通过表达解决不平等问题
  • 批准号:
    AH/Z505341/1
  • 财政年份:
    2024
  • 资助金额:
    $ 33.54万
  • 项目类别:
    Research Grant
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了