Development of a bedside diagnostic tool to determine low levels of free haemoglobin in whole blood

开发床边诊断工具以确定全血中低水平的游离血红蛋白

• 批准号: 2278913
• 金额: --
• 依托单位: Imperial College London
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2019
• 资助国家: 英国
• 起止时间: 2019 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2278913
• 关键词: Development; bedside; diagnostic; tool; determine

Haemoglobin (Hb) is predominantly localised within the cellular compartment of red blood cells. However, traumatic injuries, surgery and some disease states (e.g. sickle cell, thalassemia and malaria) cause red cell rupture/haemolysis which releases free Hb into the circulation. Endogenous protection is afforded by the Hb binding and removal protein haptoglobin (Ha), but this reserve is rapidly overwhelmed. Free Hb has recently been implicated in kidney failures, infection, sepsis, acute respiratory distress syndrome (ARDS), hypertension and pulmonary arterial hypertension. Thus there is an emerging need to develop a sensitive and rapid, bedside, free Hb quantitation method to help inform diagnosis and clinical management of patients. In this project, we will explore two potential solutions to this challenge one based on electrochemical detection and the other spectrophotometry.

血红蛋白（Hb）主要位于红细胞的细胞室中。然而，创伤性损伤、手术和一些疾病状态（例如镰状细胞、地中海贫血和疟疾）引起红细胞破裂/溶血，其将游离Hb释放到循环中。内源性保护由Hb结合和去除蛋白结合珠蛋白（Ha）提供，但这种储备很快就会被淹没。游离Hb最近被认为与肾衰竭、感染、败血症、急性呼吸窘迫综合征（ARDS）、高血压和肺动脉高压有关。因此，迫切需要开发一种灵敏且快速的床边游离Hb定量方法，以帮助为患者的诊断和临床管理提供信息。在这个项目中，我们将探索两种潜在的解决方案，一种是基于电化学检测，另一种是基于分光光度法。