Development of novel diagnostics for African non-falciparum malaria
非洲非恶性疟疾新型诊断方法的开发
基本信息
- 批准号:10057106
- 负责人:
- 金额:$ 18.94万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2020
- 资助国家:美国
- 起止时间:2020-06-30 至 2022-05-31
- 项目状态:已结题
- 来源:
- 关键词:AddressAffectAfricaAfrica South of the SaharaAfricanAntigensArchivesBedside TestingsBenchmarkingBiological AssayBloodCare Technology PointsCause of DeathCessation of lifeCharacteristicsClinicalClinical SensitivityClustered Regularly Interspaced Short Palindromic RepeatsCountryDemocratic Republic of the CongoDengue VirusDetectionDevelopmentDevicesDiagnosisDiagnosticDiagnostic testsDropsEpidemiologyEvaluationFalciparum MalariaFingersFluorescenceGenerationsHRP-2 proteinHumanInfectionLaboratoriesLactate DehydrogenaseLateralMalariaMethodologyMethodsModernizationMutationNucleic AcidsParasitesPathogen detectionPerformancePlasmodiumPlasmodium falciparumPlasmodium malariaePlasmodium ovalePlasmodium vivaxPolymerasePrevalenceReactionReporterReportingResearch PersonnelResourcesRibonucleasesSamplingSensitivity and SpecificitySignal TransductionSiteTanzaniaTechnologyTestingTherapeuticTimeTrainingTranslatingWorkWorld Health Organizationamplification detectionbasecostdensityexperiencefield studyhandheld equipmentimprovedmalaria infectionmortalitymultiplex detectionmutantnew technologynext generationnovelnovel diagnosticsnucleic acid detectionpathogenpoint of careprogramsrecombinasetrend
项目摘要
PROJECT SUMMARY
While malaria deaths in Africa due to Plasmodium falciparum have fallen over the last decade, the proportion
of malaria cases due to non-falciparum species is rising. Improved diagnostics are needed to respond to this
changing epidemiology. Yet current “test and treat” strategies in Africa rely upon rapid diagnostic tests
(RDTs) that do not reliably detect most non-falciparum infections and may be compromised by histidine-rich
protein 2- (PfHRP2-) negative parasites. We propose to develop and compare novel recombinase polymerase
amplification- (RPA-) and CRISPR-based diagnostic platforms that have the potential to launch the next
generation of RDTs, capable of sensitive multiplexed detection of all Plasmodium species. We have already
successfully adapted RPA and a new technology called SHERLOCK (Specific High Sensitivity Enzymatic
Reporter Unlocking) for malaria, reaching limits of detection on par with real-time PCR. We now propose to
tackle the more difficult detection of Plasmodium malariae and Plasmodium ovale. Our experience in
diagnostic development and evaluation, expertise in non-falciparum and low-density malaria infections, and
collaborative relationship with multiple in-country African investigators make us uniquely suited to pioneer this
new technology. In Aim 1, we will develop and optimize P. malariae and P. ovale RPA and SHERLOCK
assays with enhanced sensitivity and robustness, utilizing a range of strategies that have previously allowed
streamlined, multiplexed detection of multiple pathogens. We will detect target nucleic acids using a
multiplexed lateral flow device for RPA and simple fluorimeter for SHERLOCK. Sensitivity and specificity of
the new assays will be benchmarked using a large sample bank of archived isolates from the Democratic
Republic of the Congo and Tanzania. In Aim 2, we will field test the best performing RPA or SHERLOCK
assays in Bagamoyo, Tanzania. We will determine their sensitivity and compare their performance to current
RDTs, as well as real-time PCR. These studies will leverage promising new technology for pathogen
detection to provide a low-cost platform for the study of non-falciparum malaria in low-resource settings. If
successful, they will lay the groundwork for development of species-specific, sensitive RDTs capable of
multiplexed detection of both P. falciparum and non-falciparum malaria in Africa.
项目总结
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Jessica Lin其他文献
Jessica Lin的其他文献
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{{ truncateString('Jessica Lin', 18)}}的其他基金
Does treating low density malaria infections reduce malaria transmission?
治疗低密度疟疾感染是否可以减少疟疾传播?
- 批准号:
10574796 - 财政年份:2023
- 资助金额:
$ 18.94万 - 项目类别:
Development of novel diagnostics for African non-falciparum malaria
非洲非恶性疟疾新型诊断方法的开发
- 批准号:
10206017 - 财政年份:2020
- 资助金额:
$ 18.94万 - 项目类别:
Determinants of malaria transmission by submicroscopic gametocytemia
亚显微配子体血症传播疟疾的决定因素
- 批准号:
9926215 - 财政年份:2018
- 资助金额:
$ 18.94万 - 项目类别:
Determinants of malaria transmission by submicroscopic gametocytemia
亚显微配子体血症传播疟疾的决定因素
- 批准号:
10400098 - 财政年份:2018
- 资助金额:
$ 18.94万 - 项目类别:
Determinants of malaria transmission by submicroscopic gametocytemia
亚显微配子体血症传播疟疾的决定因素
- 批准号:
10189493 - 财政年份:2018
- 资助金额:
$ 18.94万 - 项目类别:
Genetic determinants of Plasmodium vivax relapse
间日疟原虫复发的遗传决定因素
- 批准号:
8679282 - 财政年份:2014
- 资助金额:
$ 18.94万 - 项目类别:
Genetic determinants of Plasmodium vivax relapse
间日疟原虫复发的遗传决定因素
- 批准号:
8991706 - 财政年份:2014
- 资助金额:
$ 18.94万 - 项目类别:
Genetic determinants of Plasmodium vivax relapse
间日疟原虫复发的遗传决定因素
- 批准号:
9222696 - 财政年份:2014
- 资助金额:
$ 18.94万 - 项目类别:
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