Exercise & influenza immunity:Psychoneuroendocrine Model

描述（由申请方提供）：拟定研究的目的是 研究定期适度运动对免疫系统的影响。 对老年人流感疫苗接种的反应;并确定是否 运动免疫调节的机制涉及心理社会因素 和/或神经内分泌适应。老年人的发病率更高， 和流感感染的严重性，以及降低的疫苗效力。我们 假设运动会增强对疫苗的免疫反应， 改善社会心理状态。共有72名年龄> 65岁的男性和女性将被 分配到运动组（EX），n=36，或对照组（CON），n=36。额外 32例受试者（n= 16 EX，n=16 CON）目前接受非选择性 将招募β-肾上腺素能受体拮抗剂来检验这一假设 运动的免疫调节作用是通过儿茶酚胺介导的， β-肾上腺素能受体的激活。受试者将接种以下疫苗： 流感疫苗，并将随机分配参加 定期剧烈有氧运动计划（EX）或灵活性计划（CON） 一年的将在免疫前和免疫后采集血样。年底 一年后，将再次对受试者进行免疫接种，并采集血样 免疫前和免疫后最多6个月。心理社会措施 抑郁，感知压力，社会支持，积极/消极影响， 在整个研究过程中，将多次评估日常麻烦/提升。的 主要免疫结局指标为：抗流感IgG和IgG 1，流感 特异性外周血单个核细胞增殖与细胞因子 （白细胞介素（IL）-2，IL-10，干扰素（IFN）-γ）产生，细胞毒性T细胞 淋巴细胞（CTL）功能，以及细胞内流感特异性IL-2，IL-β 0， IFN-γ，在CD 4+、CD 8+、CD 45 RA+和CD 45 RO+细胞中。增长曲线建模将 用于分析治疗条件与 随着时间的推移免疫活性的测量。这项研究的结果可能 从临床和基础研究的角度来看都具有相关性，如果中等 运动与增强抗流感免疫力有关， 保护免受感染。

DESCRIPTION (provided by applicant): The purpose of the proposed study is to investigate the effects of a regular, moderate exercise program on the immune response to influenza immunization in older adults; and to determine whether the mechanisms of the exercise-induced immunomodulation involve psychosocial and/or neuroendocrine adaptations. Older adults experience a greater incidence and severity of influenza infection, as well as reduced vaccine efficacy. We hypothesize that exercise will enhance immune response to the vaccine and improve psychosocial state. A total of 72 men and women > age 65 will be assigned to an exercise (EX), n=36, or control (CON) group, n=36. An additional 32 subjects (n= 16 EX, n=16 CON) currently treated with non-selective beta-adrenergic receptor antagonists will be recruited to test the hypothesis that the immunomodulatory effects of exercise are mediated via catecholamine activation of beta-adrenergic receptors. Subjects will be immunized with influenza vaccine and will be randomly assigned to participate in either a regular vigorous aerobic exercise program (EX) or a flexibility program (CON) for one year. Blood samples will be taken pre and post-immunization. At the end of one year, subjects will be immunized again and blood samples will be taken pre-immunization and up to 6 months post-immunization. Psychosocial measures of depression, perceived stress, social support, positive/negative affect, and daily hassles/uplifts will be assessed numerous times throughout the study. The primary immune outcome measures are: anti-influenza IgG and IgG1, influenza specific peripheral blood mononuclear cell proliferation and cytokine (interleukin (lL)-2, IL-10, interferon (IFN)-gamma) production, cytotoxic T lymphocyte (CTL) function, and intracellular influenza-specific IL-2, IL-b 0, IFN-gamma, in CD4+, CD8+, CD45RA+ and CD45RO+ cells. Growth curve modeling will be used to analyze the relationship between treatment condition and changes in the measures of immunocompetence over time. The findings from this study may have relevance from both a clinical and basic research perspective, if moderate exercise is associated with enhanced anti-influenza immunity and improved protection from infection.