Exercise & influenza immunity:Psychoneuroendocrine Model

描述(申请人提供)：建议研究的目的是 调查有规律的、适度的运动计划对免疫系统的影响 老年人对流感疫苗的反应；并确定 运动性免疫调节的机制涉及心理社会 和/或神经内分泌适应。老年人的发病率更高 以及流感感染的严重性，以及疫苗效力的降低。我们 假设运动将增强对疫苗的免疫反应，并 改善心理社会状态。共有72名年龄为65岁的男性和女性将参加 被分配到运动组(EX)，n=36，或对照组(Con)，n=36。一项额外的 32名受试者(n=16名EX，n=16名CON)目前接受非选择性药物治疗 将招募β-肾上腺素能受体拮抗剂来检验这一假设 运动的免疫调节作用是通过儿茶酚胺介导的 激活β-肾上腺素能受体。受试者将接种疫苗 流感疫苗，并将被随机分配参加 常规的剧烈有氧运动计划(EX)或柔韧性计划(CON) 一年的时间。将在免疫前和免疫后采集血样。在最后 一年后，受试者将再次接种疫苗，并采集血样 免疫前和免疫后最多6个月。心理社会测量 抑郁、感受到的压力、社会支持、积极/消极情绪，以及 在整个研究过程中，将多次评估日常的麻烦/提升。这个 主要免疫结果指标为：抗流感免疫球蛋白和免疫球蛋白1、流感 特异性外周血单个核细胞增殖与细胞因子 (IL-2、IL-10、干扰素-γ)产生，细胞毒T细胞 淋巴细胞(CTL)功能和细胞内流感特异性IL-2、IL-b 0、 CD4、CD8、CD45RA和CD45RO细胞表达干扰素-γ。增长曲线建模将 用来分析治疗情况与病情变化的关系 随着时间推移免疫能力的测量。这项研究的发现可能 从临床和基础研究的角度来看都有相关性，如果适度的话 运动与增强抗流感免疫力和改善 防止感染。

DESCRIPTION (provided by applicant): The purpose of the proposed study is to investigate the effects of a regular, moderate exercise program on the immune response to influenza immunization in older adults; and to determine whether the mechanisms of the exercise-induced immunomodulation involve psychosocial and/or neuroendocrine adaptations. Older adults experience a greater incidence and severity of influenza infection, as well as reduced vaccine efficacy. We hypothesize that exercise will enhance immune response to the vaccine and improve psychosocial state. A total of 72 men and women > age 65 will be assigned to an exercise (EX), n=36, or control (CON) group, n=36. An additional 32 subjects (n= 16 EX, n=16 CON) currently treated with non-selective beta-adrenergic receptor antagonists will be recruited to test the hypothesis that the immunomodulatory effects of exercise are mediated via catecholamine activation of beta-adrenergic receptors. Subjects will be immunized with influenza vaccine and will be randomly assigned to participate in either a regular vigorous aerobic exercise program (EX) or a flexibility program (CON) for one year. Blood samples will be taken pre and post-immunization. At the end of one year, subjects will be immunized again and blood samples will be taken pre-immunization and up to 6 months post-immunization. Psychosocial measures of depression, perceived stress, social support, positive/negative affect, and daily hassles/uplifts will be assessed numerous times throughout the study. The primary immune outcome measures are: anti-influenza IgG and IgG1, influenza specific peripheral blood mononuclear cell proliferation and cytokine (interleukin (lL)-2, IL-10, interferon (IFN)-gamma) production, cytotoxic T lymphocyte (CTL) function, and intracellular influenza-specific IL-2, IL-b 0, IFN-gamma, in CD4+, CD8+, CD45RA+ and CD45RO+ cells. Growth curve modeling will be used to analyze the relationship between treatment condition and changes in the measures of immunocompetence over time. The findings from this study may have relevance from both a clinical and basic research perspective, if moderate exercise is associated with enhanced anti-influenza immunity and improved protection from infection.