Evaluation of nitrated-chemokines to monitor ischaemia reperfusion injury in organ transplantation

提高器官移植的数量和质量是一个尚未得到满足的需求。缺氧和血液供应恢复后的压力会导致分子的产生，这些分子可以改变参与炎症的蛋白质，称为趋化因子。这些修饰的趋化因子可能成为移植前评估器官质量的新的生物标志物。我们开发了一种新的针对修饰趋化因子的抗体；我们将量化移植患者样本中天然趋化因子与修饰趋化因子的比率。我们还将使用另一种使用质谱学的检测方法，以证实结果。这将允许移植患者分层。

There is an unmet need to improve the number and quality of organs transplanted. The stress following lack of oxygen and when blood supply is reinstated results in production of molecules which modify the proteins involved in inflammation, called chemokines. These modified chemokines could be novel biomarkers in assessing organ quality before transplantation. We have developed a novel antibody for modified-chemokine; we will quantify the ratio of native to modified-chemokine in transplant patient samples. We will also use a second detection method using mass spectrometry in order to corroborate the results. This will allow transplant patient stratification.