Evaluation of nitrated-chemokines to monitor ischaemia reperfusion injury in organ transplantation

硝化趋化因子监测器官移植缺血再灌注损伤的评价

• 批准号: 2281861
• 金额: --
• 依托单位: Newcastle University
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2019
• 资助国家: 英国
• 起止时间: 2019 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2281861
• 关键词: Evaluation; nitrated; chemokines; monitor; ischaemia

There is an unmet need to improve the number and quality of organs transplanted. The stress following lack of oxygen and when blood supply is reinstated results in production of molecules which modify the proteins involved in inflammation, called chemokines. These modified chemokines could be novel biomarkers in assessing organ quality before transplantation. We have developed a novel antibody for modified-chemokine; we will quantify the ratio of native to modified-chemokine in transplant patient samples. We will also use a second detection method using mass spectrometry in order to corroborate the results. This will allow transplant patient stratification.

改善移植器官的数量和质量的需求尚未得到满足。缺氧后的压力和血液供应恢复时会导致分子的产生，这些分子会改变与炎症有关的蛋白质，称为趋化因子。这些修饰的趋化因子可能成为移植前评估器官质量的新生物标志物。我们已经开发了一种新的修饰趋化因子的抗体，我们将量化移植患者样本中天然趋化因子与修饰趋化因子的比例。我们还将使用第二种检测方法（使用质谱法）来证实结果。这将允许移植患者分层。