Multiplex system for stem cell culture and in situ assay

用于干细胞培养和原位测定的多重系统

• 批准号: 6802428
• 负责人: Joel Voldman
• 金额: $ 15.04万
• 依托单位: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-09-30 至 2006-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6802428
• 关键词: autocrine; bioengineering /biomedical engineering; biomedical automation; cell proliferation; embryonic stem cell; immunologic assay /test; laboratory mouse; light microscopy; morphology; phenotype; pluripotent stem cells; stem cells; technology /technique development; tissue /cell culture

DESCRIPTION (provided by applicant): This project concerns the development of technology to streamline the ability to manipulate stem cells in culture and automate the acquisition of information from these cells. A system is proposed that uses microfabrication--the same technology used to make computer chips--to make large arrays of small chambers in which one can grow stem cells. The culture conditions in each of these chambers can be varied, allowing one to perform many experiments simultaneously. In addition, single cells will be positioned in these chambers using electric fields, allowing for a significant increase in the density of stem cell colonies, and thus a higher number of chambers on each chip. This microsystem will have environmental control of the chambers (temperature, pH, oxygen) and will couple to an automated microscopy system to allow for in situ analysis of cell phenotype. The complete system will have a small form factor (the size of a glass slide) and be inexpensive enough to be widely accessible. Initial studies will be performed examining the role of autocrine signaling on the maintenance of mouse embryonic stem cell phenotype, using the microfluidics to precisely deliver varying concentrations and flow rates of cytokines to the cell colonies.

描述（由申请人提供）：该项目涉及技术开发，以简化在培养中操纵干细胞的能力并自动化从这些细胞中获取信息。提出了一种系统，使用微加工-用于制造计算机芯片的相同技术-来制造可以在其中生长干细胞的小腔室的大阵列。这些腔室中的每一个中的培养条件可以变化，允许同时进行许多实验。此外，单细胞将使用电场定位在这些腔室中，从而显著增加干细胞集落的密度，从而增加每个芯片上的腔室数量。 该微系统将对腔室进行环境控制（温度、pH值、氧气），并将与自动显微镜系统耦合，以允许对细胞表型进行原位分析。完整的系统将具有小的形状因子（玻璃载玻片的大小），并且足够便宜，可以广泛使用。将进行初步研究，检查自分泌信号对维持小鼠胚胎干细胞表型的作用，使用微流体精确地将不同浓度和流速的细胞因子递送到细胞集落。