Brain H2D MRS: Implemation and Initial Applications

Brain H2D MRS：实施和初步应用

• 批准号: 6776995
• 负责人: ZHIYUE J WANG
• 金额: $ 17.87万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-08-15 至 2007-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6776995
• 关键词: adult human (21+); bioimaging /biomedical imaging; brain disorders; brain imaging /visualization /scanning; brain metabolism; branched chain aminoacid; child (0-11); diet therapy; electron spin resonance spectroscopy; galactosemias; gamma aminobutyrate; human subject; ketoacid; magnetic resonance imaging; maple syrup urine disease; metabolism disorder; noninvasive diagnosis; patient oriented research; sugar alcohols; technology /technique development

DESCRIPTION (provided by applicant) The long-term objective is to apply 2D 1H magnetic resonance spectroscopy (MRS) techniques in studies of pediatric neurological disorders. 1H MRS is valuable for evaluating neurological brain diseases. However, there are limitations in current clinical 1H MRS examinations. Most peaks are crowded in a narrow aliphatic spectral window, and numerous low concentration metabolites are overshadowed by a few metabolites present in higher levels, and much valuable information is lost. Most metabolites have coupled spins, and 2D MRS separates the peaks in a second frequency dimension, greatly increasing the information content of the data. Application of 2D MRS in clinical examinations will enhance the abilities for diagnoses and patient management, and improve the understanding of disease processes. The specific aims are: (1) to implement 2D spin-echo double-quantum MRS pulse sequences for in vivo measurement of brain metabolites in 1.5T clinical scanners: (1a) to implement a localized pulse sequence optimized for GABA measurement; (1b) to implement localized pulse sequences for general detection of metabolites; (1c) to implement whole-brain measurement pulse sequences for general detection of metabolites. (2) to assign and evaluate peaks detected in the normal brain: (2a) to acquire spectra from metabolite solutions; (2b) to acquire brain spectra and baselines due to macromolecules in a group of adult normal volunteers; (2c) to assign the in vivo peaks and measure metabolite levels, and to determine optimal pulse sequences for studies of Aim 3; (2d) to acquire age-matched control spectra from normal children. (3) to explore the utility of the 2D MRS techniques in patients between the ages of 7 and 11 years: (3a) to study the effects of the ketogenic diet (KD) on brain GABA levels in seizure patients; (3b) to measure the brain?s level of branched chain amino-acids (BCAA) and keto-acids (BCKA) in maple syrup urine disease (MSUD); (3c) to test the hypothesis that low levels of brain galactitol are present even under a lactose restrictive diet; (3d) to study unassigned, unusual spectral peaks found in routine clinical MRS examinations. A localized 1D double-quantum filtered MRS pulse sequence will be modified into several 2D double quantum MRS pulses for localized and whole brain measurement. Different pulse parameters will be used for different types of spin systems. A frequency selective coherent transfer pulse will be used for optimal detection of GABA, and a broadband coherent transfer pulse will be used for all other 2D pulse sequences. The measurement procedures will be applied to normal subjects first. MRS measured GABA level before and after initiation of KD therapy will be compared in seizure patients and correlated with response. The MRS measurement of BCAAs and BCKAs will be conducted during metabolic crisis and in normal conditions in MSUD and correlated with clinical condition and serum BCAA and urine BCKA. In galactosemia, 2D MRS will be used to look for low levels of galactitol, and the MRS results will be correlated with the urine galactitol levels. 2D MRS will also be used to characterize unusual peaks found in clinical practice.

描述（由申请人提供） 长期目标是应用2D 1H磁共振波谱（MRS） 儿科神经系统疾病研究中的技术。1H MRS有价值 用于评估神经性脑部疾病。然而，在以下方面存在局限性： 临床1H MRS检查。大多数山峰都挤在狭窄的 脂肪族光谱窗口，以及许多低浓度代谢物， 被一些更高水平的代谢物所掩盖， 信息丢失。大多数代谢物具有耦合自旋，并且2D MRS分离 在第二个频率维度的峰值，大大增加了信息 数据的内容。二维磁共振波谱在临床检查中的应用 提高诊断和病人管理能力， 了解疾病的过程。具体目标是：（1）实现2D 自旋回波双量子磁共振波谱脉冲序列在脑活体测量中应用 1.5T临床扫描仪中的代谢物：（1a）实现局部脉冲 序列优化GABA测量;（1b）实现局部脉冲 用于代谢物的一般检测的序列;（1c）实施全脑 测量脉冲序列用于代谢物的一般检测。(2)分配 并评估在正常脑中检测到的峰：（2a）从 代谢物溶液;（2b）获取脑光谱和基线， （2c）在一组成年正常志愿者中分配大分子; 体内峰值和测量代谢物水平，并确定最佳脉冲 目标3的研究序列;（2d）获得年龄匹配的对照光谱 从正常的孩子。(3)探讨二维磁共振波谱技术在 7 - 11岁的患者：（3a）研究 生酮饮食（KD）对癫痫患者脑GABA水平的影响;（3b）测量 大脑？支链氨基酸（BCAA）和酮酸（BCKA）水平 枫糖浆尿病（MSUD）;（3c）检验低水平 甚至在乳糖限制性饮食下也存在脑半乳糖醇;（3d）至 研究未分配，常规临床MRS中发现的异常谱峰 考试局部化的1D双量子滤波MRS脉冲序列将被 修改为几个二维双量子MRS脉冲，用于局部和整体 大脑测量不同的脉冲参数将用于不同的类型 的自旋系统。频率选择性相干转移脉冲将用于 GABA的最佳检测，并将使用宽带相干转移脉冲 所有其他2D脉冲序列。测量程序将适用于 正常人优先MRS测量开始前和开始后的GABA水平 将在癫痫发作患者中比较KD治疗，并与缓解相关。 代谢期间将进行BCAA和BCKAs的MRS测量 MSUD中的危象和正常情况，并与临床状况相关 血BCAA和尿BCKA。在半乳糖血症中，2D MRS将用于寻找 低水平的半乳糖醇，MRS结果将与尿液相关 半乳糖醇水平。2D MRS还将用于表征发现的异常峰 在临床实践中。

项目成果

Late proton MR spectroscopy in children after traumatic brain injury: correlation with cognitive outcomes.

创伤性脑损伤后儿童的晚期质子磁共振波谱：与认知结果的相关性。

• 发表时间: 2005
• 期刊: AJNR. American journal of neuroradiology
• 作者: Hunter,JillV;Thornton,RobertJ;Wang,ZhiyueJ;Levin,HarveyS;Roberson,Garland;Brooks,WilliamM;Swank,PaulR
• 通讯作者: Swank,PaulR