BRAIN PROTON MAGNETIC RESONANCE SPECTROSCOPY OF CANAVAN DISEASE

卡纳万病的脑质子磁共振波谱分析

• 批准号: 6495471
• 负责人: ZHIYUE J WANG
• 金额: $ 22.55万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-09-01 至 2002-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6495471
• 关键词: Mammalia; bioimaging /biomedical imaging; biomedical resource; building /facility design /renovation; female; health care; human subject; magnetic resonance imaging; male; medical rehabilitation related tag; musculoskeletal system; prosthesis

Microamperage electrical stimulation ( MENS) is a modality widely used by physical therapists for pain relief and tissue repair. Manufacturers of MENS units have claimed that increased ATP levels result from these stimulation units, though the mechanism of action is unknown and may be dependent on various stimulation paradigms. Studies have been done which establish the link between MENS and ATP synthesis in rat skin (Cheng, et. al., 1982). However, there is a dearth of information on the effect of MENS on ATP in human tissue, despite the FDA approval of MENS units and the wide use of these units in rehabilitative therapy. The purpose of this study is to examine the effect of MENS on muscle metabolism in the human masseter using Magnetic Resonance Spectroscopy (MRS). MRS can objectively evaluate the efficacy of MENS using phosphorous metabolite spectra to measure Pi, Phosphocreatine, and ATP peak areas and ratios. Temporomandibular joint disorder/dysfunction (TMJ) patients will be used in this study to help determine if the MENS units increase the rate of ATP production (which would be expected to aid in tissue repair and pain relief) in damaged or weak tissue. We hypothesize that MENS stimulation will change muscle pH and consumption of phosphorous containing compounds in the TMJ cohort but will not affect the normal population. The pilot study includes both normal and TMJ patients with varying parameters of stimulation to detect optimal levels of stimulation. Once completed, a double blind study will proceed using MRS on normal and TMJ patients with consistent stimulation parameters to measure muscle metabolite levels prior to and during exposure to MENS. Coils will be placed on both masseter muscles in order to measure changes in the metabolite levels in each muscle. Pilot data on 18 normal subjects has not shown ATP level changes with various stimulation parameters, which is in keeping with our hypothesis.

微安培电刺激（MENS）是一种广泛应用的方式， 物理治疗师用来缓解疼痛和修复组织。 MENS装置的制造商声称， 结果从这些刺激单位，虽然作用机制是 未知的并且可能依赖于各种刺激范例。 已经进行了研究，建立了MENS和ATP之间的联系 在大鼠皮肤中的合成（Cheng，et.例如，1982年）。 但有一个 缺乏关于MENS对人体组织中ATP的影响的信息， 尽管FDA批准了MENS单位并且这些单位被广泛使用 在康复治疗中。 本研究的目的是检查MENS对 人咬肌肌肉代谢的磁共振研究 波谱学（MRS）。 MRS能客观评价MENS的疗效 使用磷代谢物光谱测量Pi，磷酸肌酸， 和ATP峰面积和比率。 颞下颌关节 本研究将使用TMJ障碍/功能障碍（TMJ）患者， 确定MENS单元是否增加ATP产生的速率（其 预期有助于组织修复和疼痛缓解） 或者脆弱的组织 我们假设MENS刺激会改变 肌肉pH值和含磷化合物的消耗 TMJ队列，但不会影响正常人群。 该初步研究包括正常和TMJ患者， 改变刺激参数以检测最佳水平的 刺激. 一旦完成，将使用 正常人和颞下颌关节患者在一致刺激参数下的MRS 测量肌肉代谢物水平之前和期间暴露于 男人。 线圈将被放置在两个咬肌上，以便 测量每块肌肉中代谢物水平的变化。 导频数据 在18名正常受试者中，没有显示ATP水平的变化， 刺激参数，这与我们的假设一致。