BRAIN PROTON MAGNETIC RESONANCE SPECTROSCOPY OF CANAVAN DISEASE

微安培电刺激(MENS)是一种广泛的电刺激方式 理疗师用来缓解疼痛和修复组织的。 男士单元的制造商声称，增加的ATP水平 来自这些刺激单元的结果，尽管其作用机制是 未知，并可能依赖于各种刺激范例。 已经有研究确定了男人和三磷酸腺苷之间的联系 大鼠皮肤中的合成(cheng，et.Al.，1982)。然而，有一个 缺乏关于男人对人体组织中ATP影响的信息， 尽管FDA批准了MANS装置并广泛使用这些装置 在康复治疗中。 这项研究的目的是考察男性对健康的影响 磁共振在人咬肌肌肉代谢研究中的应用 波谱(MRS)。MRS可以客观地评价男性的疗效。 用磷代谢产物光谱测定PI、磷酸肌酸、 以及ATP峰面积和比值。颞下颌关节 障碍/功能障碍(TMJ)患者将在本研究中提供帮助 确定MENS单位是否提高了ATP的生成率(哪种 有望帮助组织修复和缓解疼痛) 或脆弱的组织。我们假设男人的刺激将会改变 肌肉的pH值和含磷化合物的消耗量 TMJ队列，但不会影响正常人群。 这项初步研究包括正常患者和患有TMJ的患者 改变刺激参数以检测最佳水平的 刺激。一旦完成，将使用以下方法进行双盲研究 刺激参数一致的正常人和TMJ患者的磁共振波谱研究 测量暴露前和暴露期间的肌肉代谢物水平 男人们。将线圈放置在两块咬肌上，以便 测量每块肌肉中代谢物水平的变化。试点数据 18例正常受试者未表现出不同程度的ATP水平变化 刺激参数，这符合我们的假设。

Microamperage electrical stimulation ( MENS) is a modality widely used by physical therapists for pain relief and tissue repair. Manufacturers of MENS units have claimed that increased ATP levels result from these stimulation units, though the mechanism of action is unknown and may be dependent on various stimulation paradigms. Studies have been done which establish the link between MENS and ATP synthesis in rat skin (Cheng, et. al., 1982). However, there is a dearth of information on the effect of MENS on ATP in human tissue, despite the FDA approval of MENS units and the wide use of these units in rehabilitative therapy. The purpose of this study is to examine the effect of MENS on muscle metabolism in the human masseter using Magnetic Resonance Spectroscopy (MRS). MRS can objectively evaluate the efficacy of MENS using phosphorous metabolite spectra to measure Pi, Phosphocreatine, and ATP peak areas and ratios. Temporomandibular joint disorder/dysfunction (TMJ) patients will be used in this study to help determine if the MENS units increase the rate of ATP production (which would be expected to aid in tissue repair and pain relief) in damaged or weak tissue. We hypothesize that MENS stimulation will change muscle pH and consumption of phosphorous containing compounds in the TMJ cohort but will not affect the normal population. The pilot study includes both normal and TMJ patients with varying parameters of stimulation to detect optimal levels of stimulation. Once completed, a double blind study will proceed using MRS on normal and TMJ patients with consistent stimulation parameters to measure muscle metabolite levels prior to and during exposure to MENS. Coils will be placed on both masseter muscles in order to measure changes in the metabolite levels in each muscle. Pilot data on 18 normal subjects has not shown ATP level changes with various stimulation parameters, which is in keeping with our hypothesis.