Dissecting the interplay between cardiomyocytes and fibroblasts using human stem cell models of disease

项目目标：1；2.开发和优化人类多能干细胞-心肌细胞（hPSC-CM）和心脏成纤维细胞的二维共培养和高分辨率电生理表型分析方法。表征心肌细胞-成纤维细胞偶联对电功能和钙处理的影响，3。利用Cas9/CRISPR技术生成表达常见肌瘤基因突变的hPSC-CM细胞系，并评估其对心肌细胞-成纤维细胞共培养中电生理和线粒体功能的影响。

Project aims: 1. Develop and optimise methodology for 2D co-culture and high resolution electrophysiological phenotyping of human pluripotent stem cell-cardiomyocytes (hPSC-CM) and cardiac fibroblasts, 2. Characterise the effect of cardiomyocyte-fibroblast coupling on electrical function and calcium handling, 3. Utilise Cas9/CRISPR technology to generate hPSC-CM lines expressing common sarcomeric gene mutations and asses their effects on electrophysiological and mitochondrial function in cardiomyocyte-fibroblast co-cultures.