Dissecting the interplay between cardiomyocytes and fibroblasts using human stem cell models of disease

使用人类疾病干细胞模型剖析心肌细胞和成纤维细胞之间的相互作用

• 批准号: 2282524
• 金额: --
• 依托单位: University of Birmingham
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2019
• 资助国家: 英国
• 起止时间: 2019 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2282524
• 关键词: Dissecting; interplay; between; cardiomyocytes; fibroblasts

Project aims: 1. Develop and optimise methodology for 2D co-culture and high resolution electrophysiological phenotyping of human pluripotent stem cell-cardiomyocytes (hPSC-CM) and cardiac fibroblasts, 2. Characterise the effect of cardiomyocyte-fibroblast coupling on electrical function and calcium handling, 3. Utilise Cas9/CRISPR technology to generate hPSC-CM lines expressing common sarcomeric gene mutations and asses their effects on electrophysiological and mitochondrial function in cardiomyocyte-fibroblast co-cultures.

项目目标：1.开发和优化人多能干细胞-心肌细胞（hPSC-CM）和心脏成纤维细胞的2D共培养和高分辨率电生理表型分析方法。表征心肌细胞-成纤维细胞偶联对电功能和钙处理的影响。利用Cas9/CRISPR技术产生表达常见肌节基因突变的hPSC-CM系，并评估其对心肌细胞-成纤维细胞共培养物中电生理和线粒体功能的影响。

项目成果

Complex Relationship Between Cardiac Fibroblasts and Cardiomyocytes in Health and Disease.

• DOI: 10.1161/jaha.120.019338
• 发表时间: 2021-03
• 期刊: Journal of the American Heart Association
• 影响因子: 5.4
• 作者: Hall C;Gehmlich K;Denning C;Pavlovic D
• 通讯作者: Pavlovic D