Alveolar Basement Membrane/Cell Interactions in the Lung

描述（由申请人提供）：肺肺泡区上皮易受损伤，如由空气毒性物质或氧化应激引起的损伤。损伤后这些细胞替代的中断/延迟导致肺泡的修复缺陷和不可逆转的功能受损。在本研究中，我们将证明肺上皮细胞和成纤维细胞之间存在一种关键的、动态的关系，这种关系决定了它们对硫酸盐细胞外基质（SECMs）和生长因子的反应和表达。这构成了一个由细胞自身修改的动态屏障，它定义了它们的选择性分工，最大化了它们的共同效率，并反映在这些调节因子表达的特定反调节中。要解决的假设是：肺泡上皮和底层成纤维细胞之间的硫酸酸化细胞外基质选择性地下调关键调节因子的生成和活性：FGF-1、-2和-7及其适配分子（fgf受体、fgf结合蛋白和特异性硫酸酸化ecm）。这些过程构成了肺泡正常、增生和病变状态下调节细胞数量、细胞功能和细胞结构排列的关键旁分泌和旁分泌机制。分离的AT2细胞将与成纤维细胞和硫酸盐酸化的ECM环境共同培养，通过外源添加或刺激过度生产（增强）或选择性抑制/修饰基因/蛋白质表达（减少）来修饰。为了确定控制关键上皮细胞-成纤维细胞相互作用的机制，将通过DNA合成、相关信号事件和选定基因/蛋白产物的表达来测量细胞对关键调节生长因子过表达和生长因子反应适配器的抑制翻译的反应。使用腺媒介介导的基因转移的全动物研究将确定在肺纤维化的体内模型中，FGF-BP或syndecan-1的过表达是否改善或改变了损伤/修复结果。这些研究的结果有望提供必要的信息，以更好地了解基本细胞-细胞，细胞- ecm在稳态中的关系，以及由损伤和/或疾病引起的肺纤维化变化的发病机制。

DESCRIPTION (provided by applicant): The epithelium of the alveolar region of the lung is susceptible to injury, such as that induced by air-borne toxicants or oxidant stress. Interruption/delay of the replacement of these cells following injury results in faulty repair and irreversibly impaired function in the alveolus. In this proposal, we will show that there exists a critical, dynamic relationship between lung epithelial cells and fibroblasts that dictate their responsiveness to and expression of sulfated extracellular matrices (SECMs) and growth factors. This constitutes a dynamic barrier, modified by the cells themselves, that defines their selective division of labor, maximizes their common efficiency, and is reflected in specific counter-regulation of expression of these modulatory factors. The hypothesis to be addressed is: The sulfated extracellular matrix between alveolar epithelium and underlying fibroblasts selectively down-regulates generation and activity of key regulatory factors: FGF-1, -2, and -7, and their adaptor molecules (FGF-receptors, FGF-binding protein, and specific sulfated ECMs). These processes constitute critical paracrine and juxtacrine mechanisms for regulating cell numbers, cell functions, and cellular architectural arrangement under normal, hyperplastic, and disease states in the alveolus. Isolated AT2 cells will be co-cultured with fibroblasts and the intervening sulfated ECM environment modified by either exogenous addition or stimulated overproduction (enhancement) or selective inhibition/modification of gene/protein expression (reduction). To define the mechanisms that control critical epithelial cell-fibroblast interactions, cellular responses to over expression of key regulatory growth factors and inhibited translation of growth factor response adapters will be measured by DNA synthesis, relevant signaling events, and expression of selected gene/protein products. Whole animal studies using adenovector-mediated gene transfer will determine if over expression of FGF-BP or syndecan-1 improves or alters injury/repair outcomes in an in vivo model of pulmonary fibrosis. Results of these studies are expected to provide essential information needed to better understand basic cell-cell, celI-ECM relationships in homeostasis, as well as the mechanisms that steer the pathogenesis of fibrogenic change in the lung because of injury and/or disease.