Phospholipase D in phototransduction and cellularization

描述(申请人提供)：我们研究的长期目标是了解磷脂酶D(PLD)1)调节对光转导至关重要的G蛋白偶联信号级联的分子机制，2)在果蝇细胞化过程中介导高尔基体衍生的小泡重定向用于膜生物发生。PLD水解磷脂酰胆碱产生脂质信使，磷脂酸(PA)。在哺乳动物系统中，PLD具有多种功能，包括囊泡运输、膜形成、细胞骨架重排和胞吐作用。在酵母中，PLD在减数分裂前孢子膜的形成过程中起着关键的作用。这一建议的具体目的如下：1)确定dPLD在眼睛中的亚细胞定位，并通过功能得失分析探讨其作用；2)利用突变互补从基因上剖析dPLD在光转导中的功能；3)利用标记共定位、突变互补和功能缺失分析来确定PLD在细胞化中的功能。

DESCRIPTION (provided by applicant): The long-term goal of our research is to understand the molecular mechanisms by which phospholipase D (PLD) 1) regulates the G-protein coupled signaling cascade critical for phototransduction and 2) mediates the redirection of Golgi-derived vesicles for membrane biogenesis during cellularization in Drosophila melanogaster. PLD hydrolyzes phosphatidylcholine to generate the lipid messenger, phosphatidic acid (PA). In mammalian systems, PLD has diverse functions, including vesicle trafficking, membrane formation, cytoskeletal rearrangements, and exocytosis. In yeast, PLD plays a critical role in membrane biogenesis during meiotic prospore membrane formation. The Specific Aims of this proposal are as follows: 1) determine the sub-cellular localization of dPLD in the eye and probe its role through gain- and loss-of-function analysis, 2) use mutant complementation to dissect genetically the function of dPLD in phototransduction, and 3) use marker co-localization, mutant complementation, and loss-of-function analysis to determine the function of PLD in cellularization.