Molecular Neurodevelopment: An In Vivo 31P-1H MRSI Study

分子神经发育：体内 31P-1H MRSI 研究

• 批准号: 6685201
• 负责人: JAY W PETTEGREW
• 金额: $ 55.69万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-02-01 至 2006-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6685201
• 关键词: adolescence (12-20); age difference; bioimaging /biomedical imaging; brain imaging /visualization /scanning; brain mapping; brain metabolism; clinical research; developmental neurobiology; gender difference; hydrogen ions; information systems; longitudinal human study; magnetic resonance imaging; middle childhood (6-11); morphometry; neuropsychological tests; nuclear magnetic resonance spectroscopy; phosphorus; phosphorus metabolism; technology /technique development

The goal of this project is to acquire a gender- balanced, neurodevelopmental 31P and 1H magnetic resonance spectroscopic imaging (31P-1H MRSI) molecular and metabolic normative database from a representative population of normal children and adolescents ages 6-18 years. The molecular/metabolic values for each volume of interest (voxel) will be correlated with the percent grey matter, white matter and cerebral spinal fluid (CSF) in the same voxel. The individual voxels will include the (right and left) prefrontal cortex, superior temporal cortex, inferior parietal cortex, occipital cortex, basal ganglia, centrum semiovale and thalamus. These areas are chosen so as to include areas of primary sensory (occipital and part of superior temporal cortices), heteromodal association (prefrontal, inferior parietal, and part of superior temporal cortices), subcortical grey (basal ganglia and thalamus) and white matter (centrum semiovale). Examining these areas as a function of age and gender will enable us to determine the effects of normal neurodevelopment on the molecular/metabolic measures of brain areas with different functions. 31P MRS provides measures of membrane phospholipid and high-energy phosphate metabolism and 1H MRS provides additional measures of glutamate, glutamine, taurine, N-acetylaspartate (NAA), a potential marker of neuronal functional integrity, and myo- inositol, a potential glial marker. Subjects will have a standardized neurodevelopmental test battery to assure the cohort is representative of normal cognitive development, and will have yearly pediatric physical examinations, including Tanner staging. The standardized neurodevelopmental test battery will be completed at entry, 2, and 4 years. Longitudinal 31P-1H MRSI and single voxel 1H MRS follow-up of the subjects yearly for 4 years after entry into study will be used to generate neurodevelopmental curves for brain/metabolic measures.

该项目的目的是从6-18岁的正常儿童和青少年的代表性人群中获取性别平衡，神经发育31p和1H磁共振光谱成像（31p-1h MRSI）分子和代谢规范数据库。 每次兴趣量的分子/代谢值（体素）将与同一体素中的灰质百分比，白质和脑脊髓液（CSF）相关。 单个体素将包括（右和左）前额叶皮层，上颞皮层，下顶皮层，枕皮层，基底神经节，中心神经节，半腔和丘脑。 选择这些区域，以包括主要感觉区域（枕骨和颞上皮的一部分），异源缔合（前额叶，下层壁和上等颞皮层），皮层皮层灰色（基底神经节和丘脑）和白蛋白（Centrum半决赛）。 检查这些区域是年龄和性别的函数，将使我们能够确定正常神经发育对具有不同功能的大脑区域的分子/代谢测量的影响。 31p MRS提供了膜磷脂和高能磷酸代谢的度量，1H MRS提供了谷氨酸，谷氨酰胺，牛磺酸，N-乙酰基天冬酸酯（NAA）的其他方法，这是神经元功能完整性的潜在标记，以及肌元素的肌醇，潜在的GLIAL，一种潜在的Glial标记。 受试者将具有标准化的神经发育测试电池，以确保该队列代表正常的认知发展，并将每年进行小儿体格检查，包括Tanner分期。标准化的神经发育测试电池将在入口，2和4年完成。 进入研究后4年的纵向31p-1h MRSI和单个体素MRS 1H MRS随访4年，用于生成神经发育曲线，以进行脑/代谢措施。