Molecular Neurodevelopment: An In Vivo 31P-1H MRSI Study

分子神经发育：体内 31P-1H MRSI 研究

• 批准号: 6819710
• 负责人: JAY W PETTEGREW
• 金额: $ 56.17万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-02-01 至 2006-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6819710
• 关键词: adolescence (12-20); age difference; bioimaging /biomedical imaging; brain imaging /visualization /scanning; brain mapping; brain metabolism; clinical research; developmental neurobiology; gender difference; hydrogen ions; information systems; longitudinal human study; magnetic resonance imaging; middle childhood (6-11); morphometry; neuropsychological tests; nuclear magnetic resonance spectroscopy; phosphorus; phosphorus metabolism; technology /technique development

The goal of this project is to acquire a gender- balanced, neurodevelopmental 31P and 1H magnetic resonance spectroscopic imaging (31P-1H MRSI) molecular and metabolic normative database from a representative population of normal children and adolescents ages 6-18 years. The molecular/metabolic values for each volume of interest (voxel) will be correlated with the percent grey matter, white matter and cerebral spinal fluid (CSF) in the same voxel. The individual voxels will include the (right and left) prefrontal cortex, superior temporal cortex, inferior parietal cortex, occipital cortex, basal ganglia, centrum semiovale and thalamus. These areas are chosen so as to include areas of primary sensory (occipital and part of superior temporal cortices), heteromodal association (prefrontal, inferior parietal, and part of superior temporal cortices), subcortical grey (basal ganglia and thalamus) and white matter (centrum semiovale). Examining these areas as a function of age and gender will enable us to determine the effects of normal neurodevelopment on the molecular/metabolic measures of brain areas with different functions. 31P MRS provides measures of membrane phospholipid and high-energy phosphate metabolism and 1H MRS provides additional measures of glutamate, glutamine, taurine, N-acetylaspartate (NAA), a potential marker of neuronal functional integrity, and myo- inositol, a potential glial marker. Subjects will have a standardized neurodevelopmental test battery to assure the cohort is representative of normal cognitive development, and will have yearly pediatric physical examinations, including Tanner staging. The standardized neurodevelopmental test battery will be completed at entry, 2, and 4 years. Longitudinal 31P-1H MRSI and single voxel 1H MRS follow-up of the subjects yearly for 4 years after entry into study will be used to generate neurodevelopmental curves for brain/metabolic measures.

该项目的目标是从 6-18 岁正常儿童和青少年的代表性人群中获取性别平衡、神经发育的 31P 和 1H 磁共振波谱成像 (31P-1H MRSI) 分子和代谢规范数据库。 每个感兴趣体积（体素）的分子/代谢值将与同一体素中灰质、白质和脑脊液（CSF）的百分比相关。 各个体素将包括（右和左）前额皮质、上颞叶皮质、下顶叶皮质、枕叶皮质、基底神经节、半卵圆中心和丘脑。 选择这些区域是为了包括初级感觉区域（枕叶和部分上颞皮质）、异模关联区域（前额叶、下顶叶和部分上颞皮质）、皮质下灰质（基底神经节和丘脑）和白质（半卵圆中心）。 将这些区域作为年龄和性别的函数进行检查将使我们能够确定正常神经发育对具有不同功能的大脑区域的分子/代谢测量的影响。 31P MRS 提供膜磷脂和高能磷酸盐代谢的测量，1H MRS 提供谷氨酸、谷氨酰胺、牛磺酸、N-乙酰天冬氨酸 (NAA)（神经元功能完整性的潜在标记）和肌醇（潜在的神经胶质标记）的附加测量。 受试者将接受标准化的神经发育测试，以确保该队列代表正常的认知发育，并将每年进行儿科体检，包括坦纳分期。标准化神经发育测试组将在入学、2 岁和 4 岁时完成。 进入研究后 4 年内，每年对受试者进行纵向 31P-1H MRSI 和单体素 1H MRS 随访，用于生成大脑/代谢测量的神经发育曲线。