LONG TERM CULTURE OF SPERMATOGONIAL STEM CELLS

精原干细胞的长期培养

• 批准号: 6722575
• 负责人: MARTIN DYM
• 金额: $ 7.15万
• 依托单位: GEORGETOWN UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-04-01 至 2004-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6722575
• 关键词: basement membrane; cell line; growth factor; laboratory mouse; laboratory rat; male; sperm; tissue /organ preservation

DESCRIPTION: This application will try to achieve the long term culture of type A spermatogonia. Three specific aims are proposed: 1) to determine the basement membrane components that are optimal for allowing long-term (longer than 1 week) survival of type A spermatogonia in culture; 2) to test the hypothesis that there is synergism between growth factors and basement membrane components in promoting long-term survival of spermatogonia; and 3) to develop immortalized cell lines of type A spermatogonia that will propagate in culture without losing their original properties.

描述：此应用程序将尝试实现的长期文化， A型精原细胞 提出了三个具体目标：1）确定 基底膜的组成部分是最佳的， 培养物中A型精原细胞的存活（超过1周）; 2）测试 生长因子与基底细胞之间存在协同作用的假说 膜成分在促进精原细胞长期存活中的作用; 3） 开发A型精原细胞永生化细胞系， 在文化中繁殖，而不会失去其原有的特性。

项目成果

Immunomagnetic isolation and long-term culture of mouse type A spermatogonia.

• DOI: 10.1002/j.1939-4640.2001.tb02230.x
• 发表时间: 2001-07
• 期刊: Journal of andrology
• 作者: K. S. V. D. Wee;Eric W. Johnson;G. Dirami;M. Dym;M. Hofmann

Establishment and characterization of neonatal mouse sertoli cell lines.

• DOI: 10.1002/j.1939-4640.2003.tb02650.x
• 发表时间: 2003-01
• 期刊: Journal of andrology
• 作者: M. Hofmann;K. S. Van Der Wee;Jamie L. Dargart;G. Dirami;L. Dettin;M. Dym

Structural characterization and expression studies of Dby and its homologs in the mouse.

Dby 及其同源物在小鼠中的结构表征和表达研究。

• DOI: 10.2164/jandrol.106.000471
• 发表时间: 2006
• 期刊: Journal of andrology
• 作者: Vong,QueenieP;Li,Yunmin;Lau,Yun-FaiChris;Dym,Martin;Rennert,OwenM;Chan,Wai-Yee
• 通讯作者: Chan,Wai-Yee

Identification of differentially expressed genes in mouse spermatogenesis.

小鼠精子发生中差异表达基因的鉴定。

• DOI: 10.1002/j.1939-4640.2003.tb03142.x
• 发表时间: 2003
• 期刊: Journal of andrology
• 作者: Pang,AlanLY;Taylor,HClaire;Johnson,Warren;Alexander,Stefanie;Chen,Yali;Su,YanA;Li,Xiaoquan;Ravindranath,Neelakanta;Dym,Martin;Rennert,OwenM;Chan,Wai-Yee
• 通讯作者: Chan,Wai-Yee