Hitachi Model H-7600-1 Electron Microscope

描述（由申请人提供）：80年代初，PI从NCRR计划获得资金购买电子显微镜，当时我们购买了JEOL 1200 EX。这台显微镜由细胞生物学系操作，已成为我们医疗中心的共享资源。不幸的是，显微镜近年来一直非常不可靠，事实上，在过去的六个月里，由于天花板洪水造成的广泛的水损害，它只是偶尔工作。虽然聚光镜和物镜都在更换，但JOEL不能排除由于最初的水损坏而导致的持续问题。因此，至关重要的是，我们尽快获得替代EM，因为乔治敦大学的一些教职员工有依赖于高质量电子显微镜的研究项目。我们希望购买日立H-7600-1型显微镜;该显微镜比我们老化的JEOL 1200 EX有许多优点和改进，这将大大提高我们执行本赠款建议书中所述项目的能力。包括在这些优点是更大的可靠性，易于操作，数字成像的部分，在电子束中增加的标本稳定性，增加的标本，可以在同一时间进行评估，增强使用形态分析，蒙太奇收集，易于调查的样品，并能够识别和返回到特定区域的标本网格。具有计算机化对准的能力，以及保持这些重要的观察因素，如一致的亮度，而不管操作KV或放大率，有利于测量样品和拍摄电子显微照片的容易性。最后，用户友好的操作和易于控制的操作大大减少了用户的疲劳，有利于多用户使用。我们认为，重要的是，这台显微镜尽快采购，使我们能够继续履行我们的赠款概述的研究项目的承诺。

DESCRIPTION (provided by applicant): In the early eighties, the PI received funds from the NCRR program to purchase an electron microscope, and at that time we bought the JEOL 1200EX. This microscope, operated by the Department of Cell Biology, has served as a shared resource at our Medical Center. Unfortunately, the microscope has been very unreliable in recent years and indeed for the past six months it has only worked sporadically due to extensive water damage resulting from a ceiling flood. Although both the condenser and objective lenses are being replaced, JOEL cannot rule out continued problems as a result of the initial water damage. It is therefore essential that we obtain a replacement EM as soon as possible since a number of faculty members at Georgetown have research projects that depend upon high quality electron microscopy. We wish to purchase the Hitachi Model H-7600-1; this microscope has many advantages and improvements over our aging JEOL 1200EX that would significantly enhance our ability to carry out the projects described in this grant proposal. Included among these advantages are greater reliability, ease of operation, digital imaging of sections, increased specimen stability in the electron beam, increased number of specimens which can be evaluated at one time, enhanced use for morphometric analysis, montage collection, ease of surveying of samples, and ability to identify and return to specific regions on specimen grids. The ability to have computerized alignment together with maintenance of such important viewing factors as consistent brightness regardless of operating Kv or magnification facilitates the ease of surveying samples and the taking of electron micrographs. Finally, the user-friendly operation and ease of control manipulation significantly reduces user fatigue and facilitates multi-user usage. We believe that it is important that this microscope be procured as soon as possible to enable us to continue to meet the commitments to the research projects as outlined in our grants.