Hitachi Model H-7600-1 Electron Microscope

描述（由申请人提供）：在八十年代初，PI收到NCRR计划的资金购买了一台电子显微镜，当时我们购买了JEOL 1200EX。这台显微镜由细胞生物系操作，是我们医疗中心的共享资源。不幸的是，这台显微镜近年来一直非常不可靠，而且在过去的六个月里，由于天花板上的洪水造成了大面积的水损坏，它只偶尔工作。虽然聚光镜和物镜都在更换，但JOEL不能排除由于最初的水损坏而继续出现问题的可能性。因此，我们必须尽快获得一台替代的电子显微镜，因为乔治城大学的许多教职员工都有依赖于高质量电子显微镜的研究项目。我们希望购买日立H-7600-1型；与我们老化的JEOL 1200EX相比，该显微镜有许多优点和改进，这将大大提高我们执行本拨款提案中所述项目的能力。这些优点包括更高的可靠性，易于操作，切片的数字成像，增加样品在电子束中的稳定性，增加了一次可以评估的样品数量，增强了形态测量分析的使用，蒙太奇收集，易于测量样品，以及识别和返回样品网格上特定区域的能力。计算机校准的能力以及保持诸如一致的亮度等重要的观察因素，无论操作Kv或放大倍数，都有助于测量样品和拍摄电子显微照片。最后，用户友好的操作和易于控制的操作，大大减少了用户的疲劳，便于多用户使用。我们认为，尽快采购这台显微镜是很重要的，这样我们才能继续履行对研究项目的承诺，正如我们在拨款中所概述的那样。

DESCRIPTION (provided by applicant): In the early eighties, the PI received funds from the NCRR program to purchase an electron microscope, and at that time we bought the JEOL 1200EX. This microscope, operated by the Department of Cell Biology, has served as a shared resource at our Medical Center. Unfortunately, the microscope has been very unreliable in recent years and indeed for the past six months it has only worked sporadically due to extensive water damage resulting from a ceiling flood. Although both the condenser and objective lenses are being replaced, JOEL cannot rule out continued problems as a result of the initial water damage. It is therefore essential that we obtain a replacement EM as soon as possible since a number of faculty members at Georgetown have research projects that depend upon high quality electron microscopy. We wish to purchase the Hitachi Model H-7600-1; this microscope has many advantages and improvements over our aging JEOL 1200EX that would significantly enhance our ability to carry out the projects described in this grant proposal. Included among these advantages are greater reliability, ease of operation, digital imaging of sections, increased specimen stability in the electron beam, increased number of specimens which can be evaluated at one time, enhanced use for morphometric analysis, montage collection, ease of surveying of samples, and ability to identify and return to specific regions on specimen grids. The ability to have computerized alignment together with maintenance of such important viewing factors as consistent brightness regardless of operating Kv or magnification facilitates the ease of surveying samples and the taking of electron micrographs. Finally, the user-friendly operation and ease of control manipulation significantly reduces user fatigue and facilitates multi-user usage. We believe that it is important that this microscope be procured as soon as possible to enable us to continue to meet the commitments to the research projects as outlined in our grants.