Hitachi Model H-7600-1 Electron Microscope

描述(申请人提供)：80年代初，PI从NCRR计划获得资金购买了一台电子显微镜，当时我们购买了JEOL 1200EX。这台显微镜由细胞生物系操作，已成为我们医疗中心的共享资源。不幸的是，近年来显微镜一直非常不可靠，事实上，在过去的六个月里，由于天花板洪水造成的大范围水损坏，它只能零星地工作。虽然聚光镜和物镜都在更换，但乔尔不能排除由于最初的水损坏而继续出现问题的可能性。因此，我们必须尽快获得替换的EM，因为乔治敦大学的许多教职员工都有依赖于高质量电子显微镜的研究项目。我们希望购买日立H-7600-1型显微镜；这款显微镜比我们老化的JEOL 1200EX有许多优势和改进，这将显著增强我们执行本赠款提案中所述项目的能力。这些优势包括更高的可靠性、易于操作、切片的数字成像、提高了样品在电子束中的稳定性、增加了可一次评估的样品数量、增强了用于形态测量分析、蒙太奇采集、样品测量的简便性，以及识别和返回样品网格上特定区域的能力。无论操作KV或放大倍数如何，都能够进行计算机对准，并保持一致的亮度等重要的观察系数，这有助于轻松地测量样品和获取电子显微照片。最后，用户友好的操作和易于控制的操作大大减少了用户的疲劳，促进了多用户使用。我们认为，重要的是尽快采购这台显微镜，使我们能够继续履行我们赠款中概述的对研究项目的承诺。

DESCRIPTION (provided by applicant): In the early eighties, the PI received funds from the NCRR program to purchase an electron microscope, and at that time we bought the JEOL 1200EX. This microscope, operated by the Department of Cell Biology, has served as a shared resource at our Medical Center. Unfortunately, the microscope has been very unreliable in recent years and indeed for the past six months it has only worked sporadically due to extensive water damage resulting from a ceiling flood. Although both the condenser and objective lenses are being replaced, JOEL cannot rule out continued problems as a result of the initial water damage. It is therefore essential that we obtain a replacement EM as soon as possible since a number of faculty members at Georgetown have research projects that depend upon high quality electron microscopy. We wish to purchase the Hitachi Model H-7600-1; this microscope has many advantages and improvements over our aging JEOL 1200EX that would significantly enhance our ability to carry out the projects described in this grant proposal. Included among these advantages are greater reliability, ease of operation, digital imaging of sections, increased specimen stability in the electron beam, increased number of specimens which can be evaluated at one time, enhanced use for morphometric analysis, montage collection, ease of surveying of samples, and ability to identify and return to specific regions on specimen grids. The ability to have computerized alignment together with maintenance of such important viewing factors as consistent brightness regardless of operating Kv or magnification facilitates the ease of surveying samples and the taking of electron micrographs. Finally, the user-friendly operation and ease of control manipulation significantly reduces user fatigue and facilitates multi-user usage. We believe that it is important that this microscope be procured as soon as possible to enable us to continue to meet the commitments to the research projects as outlined in our grants.