FACTORS AFFECTING SPERM FERTILITY

影响精子生育力的因素

• 批准号: 6603257
• 负责人: GABOR B HUSZAR
• 金额: $ 25.67万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-08-01 至 2005-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6603257
• 关键词: aneuploidy; carbohydrate receptor; cell age; clinical research; fertility; fluorescent in situ hybridization; human subject; hyaluronate; male; receptor binding; sperm; sperm analysis; sperm motility; spermatogenesis

This research is built upon three features: (a) immature sperm do not bind to a hyaluronic acid binding surface, and this serves as an initial marker for cells with impaired development; (b) building from the subset of sperm that do bind [apparently normal in that aspect], it is possible to analyze for other potential abnormalities; and (c) taken together, any sperm that is shown to be absent of abnormalities is a "best" candidate for intracytoplasmic sperm injection (ICSI). The "other potential abnormalities" include: presence of an abnormal protein termed CP64; shorter tail length; DNA breakdown; and chromosomal aneuploidy. Together these could form an important diagnostic test. From this background, specific aims are developed, namely: Aim #1 - use of the hyaluronic bead binding to trap sperm for further detailed analysis; Aim #2 - analysis of tail length as a simple morphologic detection of sperm immaturity; Aim #3 - analysis of chromosomal abnormalities via comparison of bound vs. non-bound cells; and Aim #4 - extension of the above to testicular biopsies and analysis of spherical spermatids.

这项研究是建立在三个特点：（a）不成熟的精子做 不与透明质酸结合表面结合，这作为初始的 发育受损细胞的标志物;（B）从以下亚组构建 精子结合[在这方面明显正常]，有可能 分析其他潜在的异常;和（c）一起，任何精子 被证明没有异常的是“最佳”候选者， 卵胞浆内单精子注射（ICSI）。“其他潜在异常” 包括：称为CP 64异常蛋白质的存在;较短的尾长; DNA 分解;和染色体非整倍性。这些共同构成了一个重要的 诊断测试在这一背景下，制定了具体目标，即： #1 -使用透明质酸珠结合来捕获精子，以进一步详细说明 分析;目的#2 -尾长分析作为一种简单的形态学检测， 精子不成熟;目标#3 -通过比较分析染色体异常 结合与非结合细胞;以及目标4-将上述内容扩展至睾丸 球形精子细胞的活检和分析。