SPERM CREATINE PHOSPHOKINASE--A PROBE OF MALE FERTILITY

精子肌酸磷酸激酶--男性生育力的探针

• 批准号: 3432445
• 负责人: GABOR B HUSZAR
• 金额: $ 2.34万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-08-24 至 1995-08-23
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3432445
• 关键词: creatine kinase; enzyme activity; fertility; human subject; isozymes; longitudinal human study; male; sperm; statistics /biometry; testis disorder

This research project integrates our clinical and basic science interests and is designed to identify objective biochemical measurements in sperm that affect and predict the fertilizing potential of male factor infertility patients. We demonstrated an inverse correlation between sperm concentrations and per sperm creatine-N-phosphotransferase (CPK) activity in normospermic and oligospermic men. This suggested to us that there are biochemical differences between sperm of these two groups. We tested this hypothesis and found that CPK activities predicted fertilizing potential in fertile and infertile men. We have also discovered a five-fold difference in the relative concentrations of B-type and M-type CPK isoforms in sperm samples with a high correlation between CPK activities and M-CPK concentrations (R=0.70, p<0.001, N=159). In ongoing research, funded by an R01 grant, we pursue clinical studies focusing upon the role of CPK activity and CPK isoform ratios in the prediction of sperm quality and fertilizing potential. We are also looking at the structure, biosynthesis and immunology of sperm CPK, and searching for further biochemical probes that may reflect sperm function. The Fogarty International Research Collaboration Award will extend our clinical studies to the Szent-Gyorgyi Albert Medical School at Szeged, Hungary. We propose to carry out CK- isoform ratio measurements in four patient groups: (a) in a retrospective study of couples treated for male factor infertility; (b) in a prospective study of infertile couples; (c) in couples with suspicion of unexplained male factor infertility and (d) in oligospermic men with varicocele before and after corrective surgery. The last project will tie in with an ongoing WHO study in Szeged. Due to the proposed collaboration, the sperm quality of varicocele patients will be also evaluated with the sperm CK-isoform ratios. The Fogarty Award will enable the collaborative investigators in Szeged to participate in a new research area of basic andrology, and it will provide the infertile couples a diagnostic technology which is presently not available in Hungary.

这个研究项目整合了我们的临床和基础科学兴趣 并被设计用于识别精子中的客观生化测量 影响和预测男性因素的潜在性 不孕症患者 我们证明了精子之间的负相关性 浓度和每个精子肌酸-N-磷酸转移酶（CPK）活性 正常精子和少精子男性。 这向我们表明， 这两组精子之间的生化差异。 我们测试了这个 假设，并发现CPK活动预测在 有生育能力和不育的男人 我们还发现了五倍的差异 精子中B型和M型CPK亚型的相对浓度 CPK活性和M-CPK之间具有高度相关性的样本 001，N=159）。 在正在进行的研究中， R 01补助金，我们追求临床研究的重点是CPK的作用 活性和CPK同工型比率预测精子质量， 有潜力。 我们也在研究它的结构， 精子CPK的免疫学研究，寻找进一步的生化探针 这可能反映了精子的功能。 福格蒂国际研究 合作奖将把我们的临床研究扩展到圣捷尔吉 匈牙利塞格德阿尔伯特医学院。 我们建议执行CK- 在四个患者组中的同种型比率测量：（a）在回顾性研究中， 对男性因素不孕症治疗夫妇的研究;（B）前瞻性研究 对不孕夫妇的研究;（c）在怀疑原因不明的夫妇中 男性因素不育和（d）在精索静脉曲张之前的少精子男性 以及矫正手术后 最后一个项目将与正在进行的 世卫组织在塞格德的研究。 由于提议的合作，精子质量 的精索静脉曲张患者也将与精子CK亚型进行评估 比率。 福格蒂奖将使合作调查人员， 塞格德参加一个新的研究领域的基础男科，它 将为不孕夫妇提供诊断技术， 目前在匈牙利还没有。