Piezoelectric QCM for Dental Caries Research

用于龋齿研究的压电 QCM

• 批准号: 6852213
• 负责人: FENGXIA QI
• 金额: $ 11.34万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-09-28 至 2006-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6852213
• 关键词: Streptococcus mutans; adult human (21+); bacterial genetics; biofilm; biomedical equipment; biomedical equipment development; clinical research; communicable disease diagnosis; confocal scanning microscopy; dental caries; dentistry; diagnostic tests; fluid flow; gene mutation; human subject; immunologic assay /test; measurement; microorganism culture; molecular weight; oral bacteria; patient oriented research; saliva; technology /technique development

DESCRIPTION: Piezoelectric quartz crystal microbalance (QCM) is a powerful tool to measure mass accretion on surfaces in the nanogram range. In the past 10 years, it has found more and more applications in a variety of biological studies from protein-protein interaction, DNA hybridization, surface attachment and biofilm formation, to detection of pathogens in food, water, and urine samples. Nevertheless, this powerful technique has not been applied in Dentistry. In this exploratory research application, investigators from UCLA School of Dentistry (UCLA) and Chemical and Environmental Engineering Department of University of California-Riverside (UCR) are partnering to introduce this technology to Dentistry by developing an integrated system combining QCM with fluidic chamber adaptable to laser scanning confocal microscopy (LSCM). We posit that this integrated system will allow for real-time measurement of biomass accretion with simultaneous analysis of structural development during biofilm formation. It will also allow for rapid, sensitive, and specific detection of oral pathogens with simultaneous image confirmation. Two Specific Aims are proposed for this study. Aim 1 is to develop a LSCM compatible, integrated QCM/fluidic chamber system for oral biofilm studies. Aim 2 is to apply QCM for rapid detection and quantification of S. mutans in mixed cultures. The outcome of this study will be the novel development and application of the QCM technology in Dentistry, which will result in acquisition of new knowledge in Dental biofilm research and development of a novel diagnostic tool that has the potential to be developed into a chair side device for oral pathogen detection.

描述：压电石英晶体微天平 (QCM) 是测量纳克范围内表面质量吸积的强大工具。在过去的10年里，它在各种生物学研究中得到了越来越多的应用，从蛋白质-蛋白质相互作用、DNA杂交、表面附着和生物膜形成，到食品、水和尿液样本中病原体的检测。然而，这种强大的技术尚未在牙科中得到应用。在这项探索性研究应用中，加州大学洛杉矶分校牙科学院 (UCLA) 和加州大学河滨分校 (UCR) 化学与环境工程系 (UCR) 的研究人员合作，通过开发一种将 QCM 与适用于激光扫描共聚焦显微镜 (LSCM) 的流体室相结合的集成系统，将该技术引入牙科。我们认为，该集成系统将允许实时测量生物量增加，同时分析生物膜形成过程中的结构发育。它还可以通过同步图像确认来快速、灵敏和特异性地检测口腔病原体。本研究提出了两个具体目标。目标 1 是开发一种兼容 LSCM 的集成 QCM/流体室系统，用于口腔生物膜研究。目标 2 是应用 QCM 快速检测和定量混合培养物中的变形链球菌。这项研究的成果将是 QCM 技术在牙科中的新颖开发和应用，这将导致获得牙科生物膜研究的新知识，并开发一种新型诊断工具，该工具有可能开发成用于口腔病原体检测的椅边设备。