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S. mutans: Its mutacin Antibiotic

S. mutans：其突变蛋白抗生素

基本信息

批准号：
7149137
负责人：
FENGXIA QI
金额：
$ 31.25万
依托单位：
UNIVERSITY OF OKLAHOMA HLTH SCIENCES CTR
依托单位国家：
美国
项目类别：
财政年份：
2003
资助国家：
美国
起止时间：
2003-03-03 至 2008-11-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7149137
关键词：
Anabolism Antibiotics Antibodies Antibody Formation Binding Biochemical Genetics Biogenesis Biological Biological Assay Biological Models Chromosomes Clinical Condition Cultured Cells DNase-I Footprinting Dental Dental Plaque Dental caries Dentistry Detection Developed Countries Developing Countries Disease Dot Immunoblotting Drug Delivery Systems Drug Design Elements Endopeptidases Engineering Enterococcus Facility Construction Funding Category Fermentation Future Generations Gel Gene Cluster Gene Expression Gene Expression Regulation Gene Fusion Genes Genetic Genetic Engineering Genetic Techniques Genome Genus staphylococcus Goals Gram-Positive Bacteria Gram-Positive Bacterial Infections Growth Hand Human Immobilized Cells Immunoassay Knowledge Lactococcus lactis Maps Measures Modification Molecular Multi-Drug Resistance Mutagenesis New York Nisin Northern Blotting Operon Organism Peptide Antibiotics Peptide Hydrolases Peptides Play Post-Translational Regulation Primer Extension Production Property Regulation Reporter Reporter Genes Research Resistance Reverse Transcriptase Polymerase Chain Reaction Role Screening procedure Site-Directed Mutagenesis Solubility Specificity Streptococcus mutans Structure Structure-Activity Relationship System Testing Time Trans-Activators Universities Variant Virulence Western Blotting Work antimicrobial antimicrobial drug antimicrobial peptide base cis acting element college design gene replacement genetic manipulation improved microbial mutacin I mutant next generation oral bacteria oral commensal pathogen promoter

项目摘要

Streptococcus mutans is the major etiologic agent responsible for dental caries, the most prevalent disease in the developed as well as developing countries. Most clinical isolates of S. mutans produce antimicrobial peptides called mutacins. Mutacins are active against a wide spectrum of gram-positive bacteria including pathogens and oral commensals. Thus, mutacin production by S. mutans may play a double role: it provides the producing strain with a competitive edge in gaining dominance in the dental plaque, leading to dental caries. On the other hand, it may protect the human host from gram-positive bacterial infections. The proposed research aims to study the genetic, biochemical and biological aspects of mutacin biogenesis and regulation with the following approaches: Aim 1, to characterize the trans-acting factors for mutacin gene regulation. Aim2, to characterize the cis-acting factors that regulate mutacin gene expression. Aim 3, to determine the structure/function of the mutacin molecule and improve mutacin's properties by genetic engineering. Aim 4, to enhance mutacin biosynthesis and improve fermentation conditions. The proposed research will have a significant impact on two fronts. The first is the alarming surge in resistance to the existing battery of antibiotics by emerging and existing pathogens, and the increasing threat of bioterrorist attack using genetically engineered pathogens resistant to all existing antibiotics. These threats underpin the importance and urgency of finding unconventional antibiotics, to which resistance has not been developed. The second front is understanding the mechanism of gene regulation for mutacin production in S. mutans. This knowledge will help design effective control measures to curb the growth and virulence of S. mutans in the dental _laque.

变形链球菌是引起龋齿最常见的主要病原体