Formulation of a novel suspending medium for immobilised culture and tissue processing

用于固定化培养和组织处理的新型悬浮介质的配制

• 批准号: 2295941
• 金额: --
• 依托单位: University of Birmingham
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2019
• 资助国家: 英国
• 起止时间: 2019 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2295941
• 关键词: Formulation; novel; suspending; medium; immobilised

Bone damage following blast or ballistic wounding results in the mechanic destruction and death of tissue at the site of injury. Surgeons debride away the tissue, removing any necrotic regions and this generally enables complete regeneration of the defect. At present, however, nobody knows the nature of damage to the tissue, as it is not possible to evaluate structure or cell response in the hard boney fragments. Our hypothesis is that high energy impact in the bone results in destruction of the osteocytic network that penetrates through it. Currently there are no methods that enable full characterisation of such fragments in anatomically relevant positions within the fracture.This project will seek to refine the supporting medium so that it is possible to maintain vital tissue in suspended culture. Doing this will allow us to evaluate the capacity of the tissue to heal in an anatomically representative environment. The student will have to manufacture a supporting phase that can mediate oxygen and nutrient transport while supporting the suspension of a relatively dense tissue [3]. The ultimate aim will be to incorporate these new materials into a novel tissue processing methodology called CLARITY, which will allow us to visual the distribution of the osteocyte network within the fracture fragments.

爆炸或弹道创伤后的骨损伤导致损伤部位组织的机械破坏和死亡。外科医生清除组织，去除任何坏死区域，这通常能够使缺损完全再生。然而，目前没有人知道组织损伤的性质，因为不可能评估硬骨碎片中的结构或细胞反应。我们的假设是，高能量的撞击会破坏穿透骨的骨细胞网络。目前，还没有方法能够在骨折的解剖学相关位置对这些碎片进行完整的表征。本项目将寻求改进支持介质，以便能够在悬浮培养中保持重要组织。这样做将使我们能够评估组织在解剖学代表性环境中愈合的能力。学生将不得不制造一个支持相，它可以介导氧气和营养物质的运输，同时支持相对致密的组织的悬浮[3]。最终目标是将这些新材料纳入一种名为CLARITY的新型组织处理方法中，这将使我们能够可视化骨折碎片内骨细胞网络的分布。