EMT: A functional and phylogenetic approach

EMT：功能和系统发育方法

• 批准号: 6791103
• 负责人: Craig Magie
• 金额: $ 4.11万
• 依托单位: UNIVERSITY OF HAWAII AT MANOA
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-09-01 至 2007-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6791103
• 关键词: Anthozoa; DNA binding protein; animal tissue; biochemical evolution; cadherins; cell cell interaction; cell differentiation; confocal scanning microscopy; embryo /fetus culture; embryogenic cleavage; epithelium; gene induction /repression; intercellular connection; invertebrate embryology; mesenchyme; mesoderm; metastasis; polymerase chain reaction; postdoctoral investigator; protein structure function; transmission electron microscopy

DESCRIPTION (provided by applicant): The epithelial to mesenchymal transition (EMT) is the process through which cells in an epithelial layer detach from that layer and become migratory. EMT is one of the most common cellular behaviors, crucial for embryonic development and the metastasis of epithelial tumors. Little is known about the regulation of EMT in non-model organisms. A functional molecular examination of this process in a phylogenetic context will identify which aspects are core components and which are species-specific modifications. Cnidarians have a simple body plan and are considered "primitive" metazoans, making them useful for comparison to "higher" organisms such as the fruit fly, mouse and human. Gastrulation in the cnidarian Nematostella vectensis involves the ingression of cells into the interior of the embryo, a process that requires cells to undergo EMT. The objectives of this proposed research are to 1) characterize the cellular basis of gastrulation and EMT in N. vectensis, and 2) determine the function of N. vectensis orthologs of snail, beta-catenin, and cell-cell unction components likely to be involved in the control of EMT to reveal whether their roles are conserved in diploblastic organism.

描述（由申请人提供）： 上皮向间充质转化（EMT）是上皮层中的细胞从该层脱离并迁移的过程。EMT是最常见的细胞行为之一，对胚胎发育和上皮性肿瘤的转移至关重要。关于EMT在非模式生物中的调节知之甚少。在系统发育的背景下，这一过程的功能分子检查将确定哪些方面是核心组件，哪些是物种特异性修饰。刺胞动物有一个简单的身体计划，被认为是“原始”的后生动物，使它们与“高等”生物如果蝇，小鼠和人类进行比较。在刺胞动物Nematostella vectensis中的原肠胚形成涉及细胞进入胚胎内部，这一过程需要细胞经历EMT。 本研究的主要目的是：1）研究N. vectensis，2）确定N的函数。vectensis的snail、β-连环蛋白和细胞-细胞功能组分的直系同源物可能参与EMT的控制，以揭示它们的作用是否在双胚生物中是保守的。