Formulation of osteogenic liposomes inspired by cell-derived vesicles

受细胞源性囊泡启发的成骨脂质体的配制

• 批准号: 2298830
• 金额: --
• 依托单位: University of Birmingham
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2019
• 资助国家: 英国
• 起止时间: 2019 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2298830
• 关键词: Formulation; osteogenic; liposomes; inspired; cell

In the last decade, the field of extracellular vesicles (EVs) has rapidly expanded and is constantly unearthing new understanding of the role these nanoparticles play in various biological processes. Within developing bone, vesicles function as vehicles for the delivery of pro-osteogenic factors and initiate early nucleation events necessary for matrix mineralisation. This project will seek to understand the cargo transferred by vesicles that modulate bone regeneration. These mechanisms will then be used as inspiration to formulate synthetic liposomes that may be incorporated into biomaterials to enhance osteogenesis. This studentship will involve delivering the following tasks for which full training will be given:1. In-vitro cell culture of osteoblasts and stem cells to produce extracellular vesicles that may then be isolated using ultracentrifugation2. Characterisation of vesicle size, cargo and lipid content using a range of different techniques3. Observe the effects of different vesicle dosages on the proliferation and mineralisation of stem cells in-vitro4. Formulate liposomes containing key vesicle proteins and characterise their physicochemical properties as well as influence of stem cell mineralisation5. Incorporate optimised liposomes into injectable hydrogel biomaterials to enable localised delivery to bone defects

在过去的十年中，细胞外囊泡（EVs）领域得到了迅速的发展，并不断发现这些纳米颗粒在各种生物过程中所起作用的新认识。在发育中的骨中，囊泡的功能是运送促成骨因子，并启动基质矿化所必需的早期成核事件。该项目将试图了解通过调节骨再生的囊泡转移的货物。然后，这些机制将作为灵感来制定合成脂质体，这些脂质体可能被纳入生物材料中以增强成骨。该学生将完成以下任务，并将接受全面的培训：成骨细胞和干细胞的体外细胞培养，产生细胞外囊泡，然后可以用超离心分离。表征囊泡大小，货物和脂质含量使用一系列不同的技术3。观察不同囊泡剂量对体外干细胞增殖和矿化的影响。配制含有关键囊泡蛋白的脂质体，描述其理化性质以及干细胞矿化的影响5。将优化的脂质体纳入可注射的水凝胶生物材料中，以实现骨缺损的局部递送