Genetic analysis of vertebrate hindbrain development

描述(由申请人提供)：在本建议书中，我试图描述 维甲酸(RA)和POU2信号在后脑的汇聚 图案化和莫特纳细胞的形成。一种推定的低到高的RA浓度 大脑中-后脑边界(MHB)和后脑/脊髓之间的梯度 边界(HSB)可能起决定后脑命运的作用。因此， 我描述了一系列实验来测试RA中是否需要POU2 通向R2中多出的Mauner神经元的途径。作为几十年的工作 已经表明，RA在许多发育过程中都是必需的，无论是超出还是超过 或缺乏会导致严重的出生缺陷，包括畸胎癌。因此， 对斑马鱼发育信号网络的分析将具有相关性 包括癌症在内的许多人类疾病。

DESCRIPTION (provided by applicant): In this proposal, I seek to characterize the convergence of retinoic acid (RA) and pou2 signaling on hindbrain patterning and mauthner cell formation. A putative low-to-high RA concentration gradient between the mid-hindbrain boundary (MHB) and the hindbrain/spinal cord boundary (HSB) might function to specify posterior hindbrain fates. Therefore, I describe a series of experiments to test whether pou2 is required in the RA pathway leading to supernumerary mauthner neurons in r2. As decades of work have shown, RA is required for many developmental processes and either excess or deficiency can cause severe birth defects including teratocarcinomas. Thus, analysis of developmental signaling networks in zebrafish will have relevance to many human diseases, including cancer.