Genetic analysis of vertebrate hindbrain development

脊椎动物后脑发育的遗传分析

• 批准号: 6622331
• 负责人: ROBERT M NISSEN
• 金额: $ 4.64万
• 依托单位: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-04-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6622331
• 关键词: Mauthner's neuron; biological signal transduction; developmental neurobiology; fibroblast growth factor; gene expression; gene mutation; genetic regulation; genetically modified animals; microarray technology; neurogenetics; postdoctoral investigator; retinoate; rhombencephalon; transcription factor; zebrafish

DESCRIPTION (provided by applicant): In this proposal, I seek to characterize the convergence of retinoic acid (RA) and pou2 signaling on hindbrain patterning and mauthner cell formation. A putative low-to-high RA concentration gradient between the mid-hindbrain boundary (MHB) and the hindbrain/spinal cord boundary (HSB) might function to specify posterior hindbrain fates. Therefore, I describe a series of experiments to test whether pou2 is required in the RA pathway leading to supernumerary mauthner neurons in r2. As decades of work have shown, RA is required for many developmental processes and either excess or deficiency can cause severe birth defects including teratocarcinomas. Thus, analysis of developmental signaling networks in zebrafish will have relevance to many human diseases, including cancer.

描述（由申请人提供）：在本提案中，我试图描述 视黄酸（RA）和pou 2信号在后脑的会聚 图案化和Mauthner细胞形成。假定的低到高RA浓度 中脑-后脑边界（MHB）和后脑/脊髓之间的梯度 边界（HSB）可能起作用，以指定后后脑的命运。因此，我们认为， 我描述了一系列的实验来测试pou 2是否是RA所必需的 导致R2中多余Mauthner神经元的通路。几十年的工作 已经表明，RA是许多发育过程所必需的， 或缺乏可导致严重的出生缺陷，包括畸胎癌。因此，在本发明中， 分析斑马鱼的发育信号网络将具有相关性 包括癌症在内的许多人类疾病。