Development of the Precerebellar System

描述（由申请人提供）：小脑功能的关键是一组 五个脑干神经元组件（核）统称为 小脑前系统这些细胞核负责传递和处理 信息从大脑皮层和脊髓流到 小脑小脑前神经元由背侧神经上皮产生 (the菱形唇），但仍停留在延髓和脑桥的腹侧 脑干为了到达它们的最终位置，这些神经元必须迁移很长时间， 在几个脑分区之间的距离充满了密集的， 突触连接的神经堆因此，研究如何发展 这一重要但很少被研究的神经复合体应该进一步阐明 成熟大脑中细胞迁移的潜在机制，以及 对我们理解小脑功能的一个重要方面有很大帮助。利用克隆 分析、基因表达的系列分析和基于重组酶的命运 绘图和诱变技术，拟议项目将侧重于 小脑前神经元的身份是在小脑内建立的机制。 菱形唇

DESCRIPTION (provided by applicant): Critical to cerebellar function is a set of five brainstem neuron assemblies (nuclei) collectively referred to as the precerebellar system. These nuclei are responsible for relaying and processing information that flows from the cerebral cortex and spinal cord to the cerebellum. Precerebellar neurons are generated from dorsal neuroepithelium (the rhombic lip) yet settle in ventral aspects of the medulla and pontine brainstem. To reach their final positions, these neurons must migrate long distances across several brain subdivisions filled with densely packed, synaptically-connected neuropile. Thus, research addressing the development of this crucial, yet rarely studied, neural complex should further elucidate mechanisms underlying cell migration in the mature brain, as well as contribute to our understanding of a critical aspect of cerebellar function. Using clonal analysis, serial analysis of gene expression, and recombinase based fate mapping and mutagenesis techniques, the proposed project will focus on the mechanisms by which precerebellar neuronal identity is established within the rhombic lip.