Control of Vulval Differentiation in C. Elegans

描述（申请人摘要）：我们使用C。女阴秀丽线虫 分化作为模型系统来研究细胞的机制， 分化和形态发生。外阴分化在C.埃莱甘斯群岛 由多种细胞信号传导和调节途径控制， 在进化上与那些通常参与控制 哺乳动物和癌症等人类疾病的发展。为下一 在此期间，我们将重点研究几个因素之间的相互作用。 监管 (1)分析ras突变抑制子定义的两个基因的作用。 (2)鉴定和表征两种转录的靶标和辅助因子 也使用大规模遗传筛选作用于RAS/MAPK下游的因子 作为分子筛选方法。 (3)分析其他几种因素对Iin-39 hox的调节作用， 分析其与其他调控途径的联系。 (4)剖析两个基因在特定外阴中的作用 形态发生事件已在拟议的 research.

DESCRIPTION (APPLICANT'S ABSTRACT): We are using C. elegans vulval differentiation as a model system to study the mechanisms of cell differentiation and morphogenesis. Vulval differentiation in C. elegans is controlled by multiple cell signaling and regulatory pathways that are evolutionarily conserved with those commonly involved in controlling development in mammals and in human diseases such as cancers. For the next granting period, we will focus our studies on interactions among several regulatory (1) Analyzing the roles of two genes defined by suppressors of a ras mutation. (2) Identifying and characterizing targets and co-factors of two transcription factors acting downstream of RAS/MAPK using large scale genetic screens as well as a molecular screening method. (3) Analyzing the regulation of Iin-39 hox by several other factors and analyzing its connections to other regulatory pathways. (4) Dissecting two genes regarding their roles in a specific vulval morphogenesis event. Significant progress has already been made on proposed research.