Microbiology Core

微生物学核心

• 批准号: 6866282
• 负责人: MARCIA METZGAR HOBBS
• 金额: $ 13.22万
• 依托单位: UNIV OF NORTH CAROLINA CHAPEL HILL
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-08-01 至 2009-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6866282
• 关键词: Chlamydia trachomatis; Haemophilus ducreyi; Neisseria gonorrhoeae; Trichomonas vaginalis; biomedical facility; clinical research; communicable disease diagnosis; cooperative study; human subject; microorganism classification; microorganism resource /registry; sample collection; tissue resource /registry; urinalysis

The Clinical Microbiology and Laboratory Core (Micro Core) in this application represents a fusion of the previously separate Microbiology Laboratory and Human Challenge Cores. The Micro Core will support specimen collection, transport, storage and testing for Dr. Cohen's studies in Project 1 and Dr. Elkins' studies in Project 2 including detection of gonorrhea, chlamydia and trichomonas in urogenital specimens and detection of genital ulcer pathogens with special emphasis on Haemophilus ducreyi from chancroid ulcers. Micro Core lab personnel perform commercially available, FDA-approved nucleic acid amplification detection assays and develop and validate in-house detection assays when no commercial product exists. A key role of the Micro Core will be to provide training and quality control and assurance plans for STD testing in Lilongwe, Malawi as required in Project 1. In addition, we will pursue an independent specific aim to characterize 1-1.ducreyi Class II strains in clinical specimens, founded on Dr. Elkins' recent discovery of an apparent second class of chancroid isolates detailed in the progress report for Project 2 in the current application. To examine the distributions of Class I and Class II H. ducreyi strains for this aim, we will collect and analyze clinical isolates from genital ulcers from geographically diverse populations including STD clinics in Malawi, Madagascar, Cameroon, Congo and China. We will characterize genotypic features associated with the two lineages by PCR amplification length polymorphisms and DNA sequence analyses of the dltA, dsrA and ncaA loci, and phenotypic traits by SDS PAGE and Western Blot for surface protein expression and antigenic characteristics, and in vitro antibiotic susceptibility testing. For Dr. Thomas' studies in Project 3, the Core will conduct human challenge experiments with Neisseria gonorrhoeae variants expressing combinations of pilE, pilC and recA mutations. The resources of the Micro Core include facilities in the UNC Department of Hospital Laboratories, the Verne S. Caviness General Clinical Research Center and in the Division of Infectious Diseases.

此应用程序中的临床微生物学和实验室核心（微核心）代表了之前单独的微生物学实验室和人类挑战核心的融合。微核心将支持科恩博士在项目 1 中的研究和埃尔金斯博士在项目 2 中的研究的样本采集、运输、储存和测试，包括检测泌尿生殖样本中的淋病、衣原体和毛滴虫，以及检测生殖器溃疡病原体，特别是软下疳溃疡中的杜克雷嗜血杆菌。 Micro Core 实验室人员执行经 FDA 批准的商业化核酸扩增检测分析，并在不存在商业产品时开发和验证内部检测分析。微核心的一个关键作用是按照项目 1 的要求，为马拉维利隆圭的 STD 检测提供培训、质量控制和保证计划。此外，我们将追求一个独立的具体目标 表征临床标本中的 1-1.ducreyi II 类菌株，该菌株基于 Elkins 博士最近发现的明显的第二类软下疳分离株，详细信息请参阅当前申请中项目 2 的进度报告。为了实现这一目的，为了检查 I 类和 II 类 H. ducreyi 菌株的分布，我们将收集并分析来自不同地理人群（包括马拉维、马达加斯加、喀麦隆、刚果和中国的 STD 诊所）的生殖器溃疡的临床分离株。我们将通过 PCR 扩增长度多态性和 dltA、dsrA 和 ncaA 位点的 DNA 序列分析来表征与两个谱系相关的基因型特征，并通过 SDS PAGE 和蛋白质印迹来表征表面蛋白表达和抗原特征以及体外抗生素敏感性测试来表征表型特征。为了托马斯博士的研究 在项目3中，核心将利用表达pilE、pilC和recA突变组合的淋病奈瑟菌变种进行人体挑战实验。微核心的资源包括北卡罗来纳大学医院实验室部、凡尔纳·S·卡维尼斯普通临床研究中心和传染病科的设施。