Clinical and Microbiology Laboratory Core

临床和微生物学实验室核心

• 批准号: 7764319
• 负责人: MARCIA METZGAR HOBBS
• 金额: $ 32.29万
• 依托单位: UNIV OF NORTH CAROLINA CHAPEL HILL
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-25 至 2014-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7764319
• 关键词: Authorization documentation; Bacterial Adhesins; Biological; Biological Markers; China; Clinic; Clinical; Clinical Microbiology; Clinical Research; Collaborations; Collection; County; Data; Detection; Development; Diagnostic; Diagnostic tests; Engineering; Epidemiology; Evaluation; Family health status; Funding; Future; Genotype; Gonorrhea; Human; Human Resources; Immune response; Infection; Institution; International; Iron; Jamaica; Knowledge; Laboratories; Madagascar; Malawi; Membrane Proteins; Neisseria gonorrhoeae; Pathogenesis; Peptidoglycan; Peru; Phase; Pilum; Prostate-Specific Antigen; Proteins; Proteomics; Protocols documentation; Public Health; Research; Research Ethics Committees; Research Infrastructure; Research Institute; Research Personnel; Research Project Grants; Resources; Role; Seminal fluid; Sexually Transmitted Diseases; Source; Specimen; Structure; System; Techniques; Technology; Testing; Trichomonas Infections; Trichomonas vaginalis; Universities; Urethra; Variant; Virginia; Washington; Wisconsin; Woman; antimicrobial; base; clinical care; design; efflux pump; human subject; lipooligosaccharide; male; men; microbial; novel diagnostics; pathogen; repository; research study; volunteer

The Clinical and Microbiology Laboratory Core (Micro Core B) supports 4 Projects in the Southeastern STI Cooperative Research Center and provides a broad base of support for other local, national and international STI research projects. We will conduct experimental human infection studies with Neisseria gonorrhoeae to determine the requirements for the gonococcal antimicrobial efflux pump, lipooligosaccharide structures and phase variation (Project 3, Shafer); pilus-associated proteins (Project 4, Thomas); iron-regulated adhesins (Project 2, Comelissen); and peptidoglycan release (Joseph Dillard, Univ. of Wisconsin) for urethral infection in men. In addition, clinical specimens from experimentally infected subjects will be collected for analysis to evaluate innate immune responses to experimental gonococcal infection (Project 5, Duncan). To facilitate the identification and characterization of iron-regulated surface proteins in N. gonorrhoeae that may function as adhesins, the Micro Core will coordinate interactions between Dr. Comelissen in Project 2 at the Virginia Commonwealth University and the UNC-Duke Michael Hooker Proteomics Center, located on the UNCChapel Hill campus. In addition, the Micro Core will serve as a repository of clinical and biological specimens from a variety of studies for future STI testing. We will continue to evaluate new diagnostic tests for sexually transmitted pathogens. In particular, we will evalute a T. vaginalis genotyping system (developed as a project in the previous NC STI-TM CRC) using recent specimens from HIV-infected women with trichomoniasis participating in an ongoing clinical study in the Gulf South STI CRC and T. vaginalis isolates from women in a recently completed study conducted by the UW STI CRC in Peru. Other activities will include the evaluation and use of tests for biomarkers of recent semen exposure, including detection of prostate-specific antigen. In addition, the Micro Core will continue to perform STI diagnostic testing in support of clinical research studies.

临床和微生物学实验室核心（微核心B）支持STI东南部的4个项目 合作研究中心，并为其他地方，国家和国际提供广泛的支持基础 科学、技术和创新研究项目。我们将进行淋病奈瑟菌的人体感染实验研究， 确定淋球菌抗菌外排泵、脂寡糖结构和 相位变化（项目3，Shafer）;菌毛相关蛋白（项目4，托马斯）;铁调节粘附素 （项目2，Comelissen）;和肽聚糖释放（Joseph Dillard，威斯康星州大学）用于尿道感染 在男人身上。此外，将采集实验感染受试者的临床标本进行分析， 评价对实验性淋球菌感染的先天免疫应答（项目5，邓肯）。以促进 N.铁调控的表面蛋白的鉴定和表征。淋病可能起作用 作为粘附素，微核心将协调弗吉尼亚州项目2中的Comelissen博士之间的相互作用。 英联邦大学和北卡罗来纳大学杜克大学迈克尔胡克蛋白质组学中心，位于北卡罗来纳大学教堂 希尔校区。此外，微核心将作为一个储存库的临床和生物 从各种研究中提取样本，用于未来的STI检测。我们将继续评估新的诊断测试 性传播的病原体特别是，我们将评估T。迷走神经基因分型系统 （作为先前NC STI-TM CRC中的一个项目开发），使用感染艾滋病毒的妇女的近期标本 参与海湾南部STI CRC和T.阴道毛 最近由UW STI CRC在秘鲁进行的一项完成的研究中，其他活动 将包括评估和使用最近精液暴露的生物标志物测试，包括检测 前列腺特异性抗原此外，微核心将继续进行STI诊断测试， 支持临床研究。