USING HUMAN HAIR TO DETERMINE LONG TERM ALCOHOL ABUSE

使用人类头发来确定长期酒精滥用情况

• 批准号: 6738887
• 负责人: CHARLES A PLATE
• 金额: $ 10万
• 依托单位: U.S. DRUG TESTING LABORATORIES, INC.
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-04-15 至 2005-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6738887
• 关键词: alcoholic beverage consumption; alcoholism /alcohol abuse; behavioral /social science research tag; biomarker; chromatography; chronic disease /disorder; clinical research; diagnosis design /evaluation; diagnostic tests; drug /alcohol abstinence; early diagnosis; esterification; esters; ethanol; free fatty acids; hair; health behavior; human subject; ionization; method development; toxin metabolism

DESCRIPTION (provided by applicant): The objective of this project is to develop a diagnostic test to assess long-term alcohol consumption based on the analysis of hair for fatty acid ethyl esters. Excessive alcohol consumption causes damage to the liver, GI tract, central nervous system, pancreas, and heart. While there are hundreds of success stories of effective intervention for problem drinking, few primary care physicians routinely screen for alcohol problems. In this program, emphasis is on the development of a biological marker for alcohol intake, using hair, so as to be initially indicative of tong term alcohol use. Repeated analyses will detect relapse drinking at an earlier stage, and throughout rehabilitation, decreasing healthcare costs dramatically. Fatty acid ethyl esters (FAEE) are present in blood, liver, and post-mortem specimens. They are formed by the esterification of ethanol with free fatty acids in the body, and contribute to ethanol-induced organ damage, through various mechanisms. FAEE's have been predominantly detected in serum and blood, and are measurable for 24 hours. However, in order to be a long-term marker of alcohol use, a specimen giving a better historical measurement of substance abuse is necessary. During Phase I, the analysis of hair for the presence of several different FAEE, both quantitatively and in profile, is proposed in order to determine drinking patterns. Hair specimens, giving an average of 3 months historical information on alcohol use, will be collected from alcoholics and teetotalers. If the analytical approach is feasible, the data produced will be able to differentiate between the two groups based on FAEE concentration and profile. Further phases of the project will include variables associated with hair color and treatments; types of shampoo and hair products used; ethnicity, age and gender. Commercialization areas of the test primarily involve recovery centers for the determination of relapse. Other areas of utility include detection of drinking in under age drivers; detection of alcohol use in pregnancy; and detection of alcohol use in potential transplant patients. The overall goal is to develop a diagnostic test to assess long-term alcohol consumption based on hair analysis for fatty acid ethyl esters.

描述(由申请人提供)：该项目的目标是开发一种诊断测试，以评估长期饮酒基于对头发的脂肪酸乙酯分析。过量饮酒会对肝脏、胃肠道、中枢神经系统、胰腺和心脏造成损害。虽然有数百个有效干预问题饮酒的成功案例，但很少有初级保健医生定期筛查酒精问题。在这个项目中，重点是开发一种酒精摄入量的生物标记，使用毛发，以便初步指示长期饮酒。重复分析将在早期阶段检测到复发饮酒，并在整个康复过程中大幅降低医疗成本。 脂肪酸乙酯(FAEE)存在于血液、肝脏和身体标本中。它们是由乙醇与体内游离脂肪酸的酯化反应形成的，并通过不同的机制促进乙醇引起的器官损伤。FAEE主要在血清和血液中检测到，并可在24小时内检测到。然而，为了成为酒精使用的长期标志，提供更好的药物滥用历史测量的样本是必要的。在第一阶段，建议对头发中存在的几种不同的FAEE进行定量和剖面分析，以确定饮酒模式。头发样本，提供了平均3个月的酒精使用历史信息，将从酗酒者和禁酒者那里收集。如果分析方法是可行的，产生的数据将能够根据FAEE的浓度和分布区分这两组人。该项目的进一步阶段将包括与头发颜色和护理相关的变量；使用的洗发水和头发产品的类型；种族、年龄和性别。该试验的商业化领域主要涉及用于确定复发的康复中心。其他有用的领域包括检测未成年司机的饮酒情况；检测怀孕期间的酒精使用情况；以及检测潜在移植患者的酒精使用情况。总体目标是开发一种基于头发脂肪酸乙酯分析的诊断测试，以评估长期饮酒情况。