900MHz NMR for Structural Biology in Chicago

芝加哥结构生物学 900MHz NMR

• 批准号: 6683150
• 负责人: PETER G.W. GETTINS
• 金额: $ 526.92万
• 依托单位: UNIVERSITY OF ILLINOIS AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-09-15 至 2008-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6683150
• 关键词: biomedical equipment resource; cryoscience; nuclear magnetic resonance spectroscopy; structural biology

DESCRIPTION (provided by applicant): The University of Illinois at Chicago (UIC), in collaboration with other major Chicago and Chicago area research institutions, namely Northwestern University, the University of Chicago, Loyola University, and the Medical College of Wisconsin, seek funds for purchase of a narrow bore 21Tesla (900MHz) NMR spectrometer and cryoprobe for carrying out, in solution, high resolution studies on proteins and protein complexes of biomedical importance that demand use of the highest available magnetic field strength. The spectrometer will be housed in a purpose-built, newly-constructed, freestanding structural biology instrumentation building on the Medical Center campus of the University of Illinois at Chicago, located just west of downtown Chicago. The spectrometer, together with a cryoprobe-equipped 800MHz spectrometer that will be installed next year, will be operated as a shared resource for all NIH-funded Chicago area investigators working on biomedically important problems. This shared resource will permit a complete transformation of protein NMR work in Chicago, from isolated campus-based investigations at relatively low field, to an interactive community of top rate investigators with access to the best equipment available. Such synergy is also likely to have a positive effect on bringing about other inter-university collaborations between Chicago universities that have previously had a more competitive than collaborative relationship. An immediate example, that also illustrates the way in which this resource will serve the current and future national scientific needs, is the development of a UIC-University of Chicago-Northwestern Research Center of Excellence in bioterrorism-related research. NMR, including the resource described here, will play a key role in this Center and in related research. As part of the facility, a resource will be established for development of procedures for intein-based segmental isotopic labeling of proteins, as well as for production of segmentally labeled DNA and RNA species.

描述(由申请人提供)：伊利诺伊大学芝加哥分校(UIC)与芝加哥和芝加哥地区的其他主要研究机构，即西北大学、芝加哥大学、洛约拉大学和威斯康星医学院合作，寻求资金购买窄口径21Tesla(900 MHz)核磁共振仪和冷冻探头，用于在溶液中对蛋白质和蛋白质复合体进行高分辨率研究，这些蛋白质和蛋白质复合体需要使用最高可用的磁场强度。光谱仪将被安置在芝加哥伊利诺伊大学医学中心校区的一座专门建造的、新建造的独立结构生物学仪器建筑中，该校园位于芝加哥市中心的西部。该光谱仪连同将于明年安装的配备冷冻探头的800 MHz光谱仪，将作为所有致力于生物医学重要问题的NIH资助的芝加哥地区调查人员的共享资源。这一共享资源将使芝加哥的蛋白质核磁共振工作彻底转变，从相对低场的孤立校园调查，转变为拥有最好设备的一流研究人员的互动社区。这种协同作用也可能对芝加哥大学之间的其他大学间合作产生积极影响，这些大学之间以前的竞争关系比合作关系更强。一个直接的例子也说明了这一资源将如何服务于当前和未来的国家科学需求，这就是UIC-芝加哥大学西北研究中心在与生物恐怖主义有关的研究方面的发展。核磁共振，包括这里描述的资源，将在该中心和相关研究中发挥关键作用。作为该设施的一部分，将建立一个资源，用于制定基于内含子的蛋白质分段同位素标记程序，以及生产分段标记的DNA和RNA物种。