900MHz NMR for Structural Biology in Chicago

芝加哥结构生物学 900MHz NMR

• 批准号: 7279979
• 负责人: PETER G.W. GETTINS
• 金额: $ 24.68万
• 依托单位: UNIVERSITY OF ILLINOIS AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-09-15 至 2009-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7279979
• 关键词: Area; Bioterrorism; Centers of Research Excellence; Chicago; Collaborations; Communities; Complex; DNA; Development; Equipment; Funding; Future; Housing; Illinois; Institution; Investigation; Label; Medical; Medical center; Play; Procedures; Production; Proteins; Purpose; RNA; Rate; Research; Research Personnel; Resolution; Resource Sharing; Resources; Role; Solutions; Universities; Wisconsin; Work; base; college; instrumentation; intein; magnetic field; structural biology

DESCRIPTION (provided by applicant): The University of Illinois at Chicago (UIC), in collaboration with other major Chicago and Chicago area research institutions, namely Northwestern University, the University of Chicago, Loyola University, and the Medical College of Wisconsin, seek funds for purchase of a narrow bore 21Tesla (900MHz) NMR spectrometer and cryoprobe for carrying out, in solution, high resolution studies on proteins and protein complexes of biomedical importance that demand use of the highest available magnetic field strength. The spectrometer will be housed in a purpose-built, newly-constructed, freestanding structural biology instrumentation building on the Medical Center campus of the University of Illinois at Chicago, located just west of downtown Chicago. The spectrometer, together with a cryoprobe-equipped 800MHz spectrometer that will be installed next year, will be operated as a shared resource for all NIH-funded Chicago area investigators working on biomedically important problems. This shared resource will permit a complete transformation of protein NMR work in Chicago, from isolated campus-based investigations at relatively low field, to an interactive community of top rate investigators with access to the best equipment available. Such synergy is also likely to have a positive effect on bringing about other inter-university collaborations between Chicago universities that have previously had a more competitive than collaborative relationship. An immediate example, that also illustrates the way in which this resource will serve the current and future national scientific needs, is the development of a UIC-University of Chicago-Northwestern Research Center of Excellence in bioterrorism-related research. NMR, including the resource described here, will play a key role in this Center and in related research. As part of the facility, a resource will be established for development of procedures for intein-based segmental isotopic labeling of proteins, as well as for production of segmentally labeled DNA and RNA species.

描述（申请人提供）：伊利诺伊大学芝加哥分校（UIC）与其他主要的芝加哥和芝加哥地区研究机构，即西北大学、芝加哥大学、洛约拉大学和威斯康星州医学院合作，寻求资金购买窄孔21特斯拉（900 MHz）NMR光谱仪和冷冻探针，用于在溶液中进行，对具有生物医学重要性的蛋白质和蛋白质复合物进行高分辨率研究，需要使用最高的可用磁场强度。光谱仪将被安置在一个专门建造的，新建的，独立的结构生物学仪器建设在医学中心校园的伊利诺伊大学芝加哥，位于市中心以西的芝加哥。该光谱仪，连同将于明年安装的配备冷冻探针的800 MHz光谱仪，将作为所有NIH资助的芝加哥地区研究生物医学重要问题的研究人员的共享资源。这种共享资源将允许蛋白质核磁共振工作在芝加哥，从孤立的校园为基础的调查在相对较低的领域，以获得最好的设备可用的顶级研究人员的互动社区的完全转变。这种协同作用也可能对芝加哥大学之间的其他大学间合作产生积极影响，这些大学之间的合作关系以前是竞争关系而不是合作关系。一个直接的例子，也说明了这种资源将服务于当前和未来的国家科学需求的方式，是芝加哥西北大学卓越研究中心的生物恐怖主义相关研究的发展。NMR，包括这里描述的资源，将在该中心和相关研究中发挥关键作用。作为该设施的一部分，将建立一个资源，用于开发基于内含肽的蛋白质片段同位素标记程序，以及生产片段标记的DNA和RNA物质。

项目成果

Orthogonal analytical methods for botanical standardization: determination of green tea catechins by qNMR and LC-MS/MS.

• DOI: 10.1016/j.jpba.2013.06.017
• 发表时间: 2014-05
• 期刊: JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
• 影响因子: 3.4
• 作者: Napolitano, Jose G.;Goedecke, Tanja;Lankin, David C.;Jaki, Birgit U.;McAlpine, James B.;Chen, Shao-Nong;Pauli, Guido F.
• 通讯作者: Pauli, Guido F.

Eucapsitrione, an anti-Mycobacterium tuberculosis anthraquinone derivative from the cultured freshwater cyanobacterium Eucapsis sp.

Eucapsitrione，一种抗结核分枝杆菌蒽醌衍生物，来自培养的淡水蓝藻 Eucapsis sp。

• DOI: 10.1021/np100299v
• 发表时间: 2010
• 期刊: Journal of natural products
• 影响因子: 5.1
• 作者: Sturdy,Megan;Krunic,Aleksej;Cho,Sanghyun;Franzblau,Scott;Orjala,Jimmy
• 通讯作者: Orjala,Jimmy

Stereochemical analysis of leubethanol, an anti-TB-active serrulatane, from Leucophyllum frutescens.

• DOI: 10.1021/np2000667
• 发表时间: 2011-09-23
• 期刊: Journal of natural products
• 影响因子: 5.1
• 作者: Molina-Salinas GM;Rivas-Galindo VM;Said-Fernández S;Lankin DC;Muñoz MA;Joseph-Nathan P;Pauli GF;Waksman N
• 通讯作者: Waksman N

Subtle Chemical Shifts Explain the NMR Fingerprints of Oligomeric Proanthocyanidins with High Dentin Biomodification Potency.

• DOI: 10.1021/acs.joc.5b01082
• 发表时间: 2015-08-07
• 期刊: The Journal of organic chemistry
• 作者: Nam JW;Phansalkar RS;Lankin DC;Bisson J;McAlpine JB;Leme AA;Vidal CM;Ramirez B;Niemitz M;Bedran-Russo A;Chen SN;Pauli GF
• 通讯作者: Pauli GF

An antimicrobial guanidine-bearing sesterterpene from the cultured cyanobacterium Scytonema sp.

来自培养的蓝藻 Scytonema sp 的抗菌含胍双倍萜。

• DOI: 10.1021/np900288x
• 发表时间: 2009
• 期刊: Journal of natural products
• 影响因子: 5.1
• 作者: Mo,Shunyan;Krunic,Aleksej;Pegan,ScottD;Franzblau,ScottG;Orjala,Jimmy
• 通讯作者: Orjala,Jimmy