Biophysical and spectroscopic analysis of skin

皮肤的生物物理和光谱分析

• 批准号: 2324708
• 金额: --
• 依托单位: University College London
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2020
• 资助国家: 英国
• 起止时间: 2020 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2324708
• 关键词: Biophysical; spectroscopic; analysis; skin

There is an urgent need to provide a molecular level insight into mechanisms of topical and transdermal active delivery as a function of anatomic site and skin health. The majority of topical preparations today typically deliver only a few per cent of active to the skin. If the reasons underlying this inefficient performance were better understood this would allow the design of better products with a lower cost. The use of Franz diffusion cell studies for studying permeation of actives through skin is well established. However, very few of these in-vitro studies have measured the amount of active which permeates through tissue from different body sites or through compromised or diseased skin sites (e.g. dandruff). Also ideally in-vivo studies should be done to confirm any findings. Such information is crucial in understanding the pathways of permeation of actives and the factors that influence this transport process. The PIs have demonstrated that new opportunities exist for profiling drug and excipients with Confocal Raman spectroscopy. The use of deuterated solvents to separate their signal from endogenous skin lipids has also been advanced by the applicants. CRS is a completely non-invasive technique and has the potential to answer fundamental questions about the healthy and diseased skin barrier. The aims and objectives of the proposed multidisciplinary work are to exploit the unique advantages of CRS to understand chemical heterogeneity of skin as a function of anatomic site and skin health. The study will elucidate these important issues in a novel manner by collecting simultaneously spatially resolved chemical information. The broader goal is to facilitate the use of CRS for skin research by revealing the underlying mechanisms of mass transport through the various strata of the skin. The accurate interpretation of spectral data generated from a chemically heterogeneous skin surface interacting with complex formulations requires careful data analysis. The application of novel chemometric data treatments will enable better and efficient data analysis compared to the traditional methods based on single peaks which have severe limitations in complex systems methods based on single peaks which have severe limitations in complex systems. The PI's have expertise in this area and have applied this before however, further work is required. As well as trainining in CRS, chemometrics and advanced data analysis the student will be trained in the use of in vitro models of drug transport and biophysical methods to interrogate skin including trans-epidermal water loss and capacitance imaging. The findings of the project are expected to lead to a better understanding of the fate of actives and excipients in skin and ultimately to the development of better topical formulations.The work to be carried out at Procter & Gamble is as follows:1. Establish in vitro skin models that are validated with biophysical methods including Trans- epidermal water loss measurement and capacitance testing. 2. Obtain skin penetration profiles via Raman spectroscopy. 3. Develop algorithms for prediction of skin uptake from in vitro data.

有一个迫切需要提供一个分子水平的洞察机制，局部和透皮活性输送作为一个功能的解剖部位和皮肤健康。目前，大多数局部制剂通常仅向皮肤递送百分之几的活性物质。如果能更好地理解这种低效性能的原因，就能以更低的成本设计出更好的产品。使用Franz扩散池研究来研究活性物质通过皮肤的渗透是公认的。然而，这些体外研究中很少测量从不同身体部位或通过受损或患病皮肤部位（例如头皮屑）渗透通过组织的活性物质的量。理想情况下，还应进行体内研究以确认任何发现。这些信息对于了解活性物质的渗透途径和影响该运输过程的因素至关重要。PI已经证明，共焦拉曼光谱法分析药物和辅料存在新的机会。申请人还提出了使用氘代溶剂将其信号与内源性皮肤脂质分离。CRS是一种完全非侵入性的技术，有可能回答有关健康和患病皮肤屏障的基本问题。拟议的多学科工作的目的和目标是利用CRS的独特优势，了解皮肤的化学异质性作为解剖部位和皮肤健康的函数。这项研究将阐明这些重要的问题，在一个新的方式，同时收集空间分辨的化学信息。更广泛的目标是通过揭示皮肤各层物质运输的潜在机制，促进CRS在皮肤研究中的应用。从化学异构的皮肤表面与复杂的配方相互作用产生的光谱数据的准确解释需要仔细的数据分析。与在复杂系统中具有严重局限性的基于单一峰的传统方法相比，新型化学计量数据处理的应用将实现更好、更高效的数据分析。PI在这方面具有专业知识，并且之前已经应用过，但是需要进一步的工作。除了CRS，化学计量学和高级数据分析的培训外，学生还将接受药物转运体外模型和生物物理方法的培训，以询问皮肤，包括经表皮水分流失和电容成像。该项目的研究结果预计将导致更好地了解活性物质和赋形剂在皮肤中的命运，并最终开发出更好的局部制剂。宝洁公司将开展的工作如下：1.建立体外皮肤模型，并通过生物物理方法进行验证，包括经表皮水分损失测量和电容测试。2.通过拉曼光谱法获得皮肤渗透曲线。3.根据体外数据开发预测皮肤吸收的算法。