Dynamics of RNA-Peptide Complex Formation

RNA-肽复合物形成的动力学

• 批准号: 6767026
• 负责人: LAWRENCE William JOHNSON
• 金额: $ 21.44万
• 依托单位: YORK COLLEGE
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-06-01 至 2008-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6767026
• 关键词: chemical structure function; conformation; fluorescence resonance energy transfer; fluorescence spectrometry; genetic regulation; genetic regulatory element; human immunodeficiency virus; intermolecular interaction; messenger RNA; molecular assembly /self assembly; molecular dynamics; thermodynamics; transcription factor; virus RNA; virus protein

There is a substantial amount of NMR and x-ray data on the structure of RNA-peptide complexes. However, there is little information about the thermodynamics and dynamics of their formation. We propose to study the dynamics and thermodynamics associated with the formation of RNA construct peptide complexes using dual color fluorescence correlation spectroscopy (DCFCS) and single pair fluorescence resonance energy transfer (spFRET). This research will focus on bovine immunodeficiency virus trans-activating region (BIV TAR), BIV trans-activator peptide (BIV Tat), human immunodeficiency virus type 1 rev response element RNA construct (HIV-1 RRE), HIV-1 regulator of viral expression peptide (HIV-1 Rev) and their respective complexes. Our specific aims are: (1) Analyze the formation of BIV Tat peptide-BIV TAR RNA complexes. (a) Thermodynamics (deltaH, deltaG, deltaS) of complex formation. (b) Thermodynamics (deltaH, deltaG, deltaS) of modified complex formation. (c) Dynamics of BIV Tat peptide beta hairpin formation. (d) Dynamics of BIV TAR RNA groove formation. (2) Analyze the formation of HIV-1 Rev peptide - RNA complexes. (a) Thermodynamics (deltaH, deltaG, deltaS) of HIV-1 Rev peptide-RNA complex formation. (b) Dynamics of HIV-1 Rev peptide - RNA complexes. (c) Dynamics of HIV-1 RRE conformational changes with and without Rev peptide. The results of this study will contribute to understanding the dynamical process of RNA-peptide binding.

关于rna -肽复合物的结构有大量的核磁共振和x射线数据。