Dynamics of RNA-Peptide Complex Formation
Dynamics of RNA-Peptide Complex Formation
批准号:
6767026
负责人:
LAWRENCE William JOHNSON
金额:
$21.44万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
2004
资助国家:
美国
项目状态:
已结题
起止时间:
2004-06-01 至 2008-05-31
关键词:
chemical structure functionconformationfluorescence resonance energy transferfluorescence spectrometrygenetic regulationgenetic regulatory elementhuman immunodeficiency virusintermolecular interactionmessenger RNAmolecular assembly /self assemblymolecular dynamicsthermodynamicstranscription factorvirus RNAvirus protein
中文摘要
关于rna -肽复合物的结构有大量的核磁共振和x射线数据。
英文摘要
There is a substantial amount of NMR and x-ray data on the structure of RNA-peptide complexes.
However, there is little information about the thermodynamics and dynamics of their formation. We propose to study the dynamics and thermodynamics associated with the formation of RNA construct peptide complexes using dual color fluorescence correlation spectroscopy (DCFCS) and single pair fluorescence resonance energy transfer (spFRET). This research will focus on bovine immunodeficiency virus trans-activating region (BIV TAR), BIV trans-activator peptide (BIV Tat), human immunodeficiency virus type 1 rev response element RNA construct (HIV-1 RRE), HIV-1 regulator of viral expression peptide (HIV-1 Rev) and their respective complexes. Our specific aims are:
(1) Analyze the formation of BIV Tat peptide-BIV TAR RNA complexes.
(a) Thermodynamics (deltaH, deltaG, deltaS) of complex formation.
(b) Thermodynamics (deltaH, deltaG, deltaS) of modified complex formation.
(c) Dynamics of BIV Tat peptide beta hairpin formation.
(d) Dynamics of BIV TAR RNA groove formation.
(2) Analyze the formation of HIV-1 Rev peptide - RNA complexes.
(a) Thermodynamics (deltaH, deltaG, deltaS) of HIV-1 Rev peptide-RNA complex formation.
(b) Dynamics of HIV-1 Rev peptide - RNA complexes.
(c) Dynamics of HIV-1 RRE conformational changes with and without Rev peptide.
The results of this study will contribute to understanding the dynamical process of RNA-peptide binding.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
ELECTRONIC SPECTROSCOPY OF SIMPLE PORPHYRINS
-
批准号:6657548
-
项目类别:
-
资助金额:$3.04万
-
财政年份:2002
-
负责人:LAWRENCE William JOHNSON
-
依托单位:
ELECTRONIC SPECTROSCOPY OF SIMPLE PORPHYRINS
-
批准号:6595206
-
项目类别:
-
资助金额:$3.04万
-
财政年份:2002
-
负责人:LAWRENCE William JOHNSON
-
依托单位:
Single Molecule Studies of Unimolecular Nanocarriers
-
批准号:6550642
-
项目类别:
-
资助金额:$5.23万
-
财政年份:2002
-
负责人:LAWRENCE William JOHNSON
-
依托单位:
ELECTRONIC SPECTROSCOPY OF SIMPLE PORPHYRINS
-
批准号:6594598
-
项目类别:
-
资助金额:$3.04万
-
财政年份:2002
-
负责人:LAWRENCE William JOHNSON
-
依托单位:
ELECTRONIC SPECTROSCOPY OF SIMPLE PORPHYRINS
-
批准号:6452771
-
项目类别:
-
资助金额:$3.15万
-
财政年份:2001
-
负责人:LAWRENCE William JOHNSON
-
依托单位:
ELECTRONIC SPECTROSCOPY OF SIMPLE PORPHYRINS
-
批准号:6478824
-
项目类别:
-
资助金额:$3.04万
-
财政年份:2001
-
负责人:LAWRENCE William JOHNSON
-
依托单位:
ELECTRONIC SPECTROSCOPY OF SIMPLE PORPHYRINS
-
批准号:6318351
-
项目类别:
-
资助金额:$3.15万
-
财政年份:2000
-
负责人:LAWRENCE William JOHNSON
-
依托单位:
ELECTRONIC SPECTROSCOPY OF SIMPLE PORPHYRINS AND BACTERIORHODOPSIN
-
批准号:6217803
-
项目类别:
-
资助金额:$20.71万
-
财政年份:1999
-
负责人:LAWRENCE William JOHNSON
-
依托单位:
ELECTRONIC SPECTROSCOPY OF SIMPLE PORPHYRINS AND BACTERIORHODOPSIN
-
批准号:6107221
-
项目类别:
-
资助金额:$20.71万
-
财政年份:1999
-
负责人:LAWRENCE William JOHNSON
-
依托单位:
ELECTRONIC SPECTROSCOPY OF SIMPLE PORPHYRINS AND BACTERIORHODOPSIN
-
批准号:6271614
-
项目类别:
-
资助金额:$18.63万
-
财政年份:1998
-
负责人:LAWRENCE William JOHNSON
-
依托单位:
MBRS/SCORE at York College
-
批准号:7253163
-
项目类别:
-
资助金额:$212.24万
-
财政年份:1997
-
负责人:LAWRENCE William JOHNSON
-
依托单位:
MBRS/SCORE at York College
-
批准号:7094127
-
项目类别:
-
资助金额:$183.93万
-
财政年份:1997
-
负责人:LAWRENCE William JOHNSON
-
依托单位:
MBRS/SCORE at York College
-
批准号:6962537
-
项目类别:
-
资助金额:$18.95万
-
财政年份:1997
-
负责人:LAWRENCE William JOHNSON
-
依托单位:
ELECTRONIC SPECTROSCOPY OF SIMPLE PORPHYRINS AND BACTERIORHODOPSIN
-
批准号:6240121
-
项目类别:
-
资助金额:$22.52万
-
财政年份:1997
-
负责人:LAWRENCE William JOHNSON
-
依托单位:
SPECTROSCOPY OF CARCINOGEN DNA ADDUCTS
-
批准号:2398104
-
项目类别:
-
资助金额:$3.67万
-
财政年份:1997
-
负责人:LAWRENCE William JOHNSON
-
依托单位:
MBRS/SCORE at York College
-
批准号:6751362
-
项目类别:
-
资助金额:$160.01万
-
财政年份:1997
-
负责人:LAWRENCE William JOHNSON
-
依托单位:
MBRS/SCORE at York College
-
批准号:6897563
-
项目类别:
-
资助金额:$129.28万
-
财政年份:1997
-
负责人:LAWRENCE William JOHNSON
-
依托单位:
MBRS-SCORE at York College
-
批准号:7137135
-
项目类别:
-
资助金额:$59.18万
-
财政年份:1997
-
负责人:LAWRENCE William JOHNSON
-
依托单位:
MBRS-SCORE at York College
-
批准号:7025456
-
项目类别:
-
资助金额:$25.5万
-
财政年份:1997
-
负责人:LAWRENCE William JOHNSON
-
依托单位:
MBRS/SCORE at York College
-
批准号:7678773
-
项目类别:
-
资助金额:$29.58万
-
财政年份:1997
-
负责人:LAWRENCE William JOHNSON
-
依托单位:
国内基金
海外基金
皮层蛋白羧基端功能的酪氨酸磷酸化调节机制及其在肿瘤细胞运动中的作用研究
-
批准号:30771126
-
项目类别:面上项目
-
资助金额:26.0万元
-
批准年份:2007
-
负责人:朱建伟
-
依托单位: