Single Molecule Studies of Unimolecular Nanocarriers

单分子纳米载体的单分子研究

• 批准号: 6550642
• 负责人: LAWRENCE William JOHNSON
• 金额: $ 5.23万
• 依托单位: RUTGERS, THE STATE UNIV OF N.J.
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-09-03 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6550642
• 关键词: amphiphilicity; cell membrane; confocal scanning microscopy; drug delivery systems; fluorescent dye /probe; liposomes; membrane permeability; microcapsule; nanotechnology; pharmacokinetics; polymers; solubility; spectrometry

DESCRIPTION (provided by applicant): Single molecule spectroscopic techniques will be used to assay the physical properties of controlled drug delivery systems, unimolecular amphiphilic nanocarriers (UAN). We will relate the physical properties of the UAN's polymer core to its ability to solubilize and deliver drug molecules. The specific aims of this project are to understand the encapsulation process that protects and solubilizes the drug molecule, and to determine the process of distribution and delivery of the drug molecule; a UAN can be used not only to solubilize a hydrophobic drug molecule, but also to protect it from environmental degradation. These studies will also help determine what factors will enable the UAN to better deliver the drug through cell membranes. The principle techniques in studying encapsulation, will be environmentally sensitive bulk and single molecule time resolved spectroscopy. A series of UAN's with modified polymer cores will be used to determine how the different flexibilities of the polymer effect solubilization of a fluorescent probe molecule. To assess the ability of a UAN to preferentially deliver drugs by permeating a membrane, actual delivery of bioactive molecules to liposomes will be dynamically imaged using scanning confocal microscopy and single molecule detection.

描述(申请人提供)：单分子光谱技术将用于分析受控药物传递系统的物理性质，单分子两亲性纳米载体(UAN)。我们将把UAN的聚合物核心的物理性质与其溶解和输送药物分子的能力联系起来。该项目的具体目标是了解保护和溶解药物分子的包埋过程，并确定药物分子的分布和输送过程；UAN不仅可以用于溶解疏水性药物分子，还可以保护其免受环境退化的影响。这些研究还将有助于确定哪些因素可以使UAN更好地通过细胞膜输送药物。研究封装的主要技术将是环境敏感的整体和单分子时间分辨光谱学。一系列具有改性聚合物核心的UAN将被用来确定聚合物的不同灵活性如何影响荧光探针分子的增溶。为了评估UAN通过透过膜优先输送药物的能力，将使用扫描共聚焦显微镜和单分子检测来动态地将生物活性分子输送到脂质体。