Bioluminescence Imaging System

生物发光成像系统

• 批准号: 6731382
• 负责人: E. DUCO JANSEN
• 金额: $ 14.81万
• 依托单位: VANDERBILT UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-04-15 至 2005-04-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6731382
• 关键词: bioimaging /biomedical imaging; bioluminescence; biomedical equipment purchase; charge coupled device camera; communicable diseases; gene expression; transplantation

DESCRIPTION (provided by applicant): A proposed Shared Instrumentation Grant (SlG) is submitted to acquire a state-of-the-art, user-friendly and fully automated Bioluminescence Imaging System (Xenogen, IVlS). The use of In Vivo Bioluminescence Imaging (IBL) has seen tremendous growth in recent years owing largely to the potential to image light emission (bioluminescence) mediated by luciferase from anywhere within small rodents. This technology may be used in studies of gene expression (using luciferase as reporter gene), infectious diseases (imaging and tracking light emitting bacteria), and transplant studies (imaging and tracking light emitting allografts). This new system is required to replace an existing home-built system based on an intensified CCD camera, which has low sensitivity to the red light used in IBL. The manual controls of all-important imaging parameters make this system difficult to manage in a multi-user environment. In fact, the existing instrument was never intended to be a shared resource, but has become the de facto user system for the University. The proposed IVlS system, which is built around a back-illuminated, back-thinned, cooled, deep depletion CCD camera, will have sensitivity levels for in vivo imaging that are 1-2 orders of magnitude better than the existing system. Further, the proposed system is designed specifically for IBL and is fully computer controlled (using Xenogen's Livinglmage software). This software package controls and logs the working distance, the lens settings (focus and fstop). The system has a heated sample stage inside the imaging chamber, as well as build-in gas anesthesia capabilities. All of these features make the IVlS system ideal for our multi-user needs. The major users of this new instrument include researchers in the fields of cancer (Coffey, Hallahan, Jansen, Lin, Matrisian), diabetes (Piston, Powers, Takahashi), lung diseases (Blackwell), and wound healing (Davidson, Jansen). The major users will account for approximately 70% of the time available on the instrument. As we expect continued growth in IBL, we have also reserved 30% time for minor and new users. The new system will be physically located in the Keck Free Electron Laser Center, which is conveniently located centrally on the Vanderbilt Campus. This multidisciplinary user center houses extensive shared spectroscopy and imaging systems that are routinely used by numerous investigators from the biological sciences, medical sciences, physical sciences, and engineering. Administration and management of the proposed system will be handled under the umbrella of the Cell Imaging Shared Resource (ClSR) which currently manages 10 shared microscopes and imaging systems, including two that are adjacent to the location of this new instrument.

描述（由申请人提供）： 一项拟议的共享仪器赠款（SLG）被提交，以获得一个最先进的，用户友好的和全自动的生物发光成像系统（Xenogen，IVIS）。近年来，体内生物发光成像（IBL）的使用出现了巨大的增长，这主要是由于在小型啮齿动物体内任何地方通过荧光素酶介导的光发射（生物发光）成像的潜力。该技术可用于基因表达的研究（使用荧光素酶作为报告基因）、感染性疾病（成像和跟踪发光细菌）和移植研究（成像和跟踪发光同种异体移植物）。这一新系统需要取代现有的基于增强型CCD摄像机的自制系统，该摄像机对IBL中使用的红光敏感度低。所有重要成像参数的手动控制使得该系统难以在多用户环境中管理。事实上，现有的仪器从来没有打算成为一种共享资源，而是成为联合国大学事实上的用户系统。所提出的IVIS系统是围绕背照式、背薄式、冷却式、深度耗尽型CCD相机构建的，其体内成像的灵敏度水平比现有系统好1-2个数量级。此外，所提出的系统是专门为IBL设计的，并且完全由计算机控制（使用Xenogen的Livinglmage软件）。该软件包控制并记录工作距离、透镜设置（焦距和光圈）。该系统在成像室内有一个加热的样品台，以及内置的气体麻醉功能。所有这些功能使IVIS系统非常适合我们的多用户需求。这种新仪器的主要用户包括癌症（Coffey，Hallahan，Jansen，Lin，Matrisian），糖尿病（Piston，Powers，Takahashi），肺部疾病（Blackwell）和伤口愈合（Davidson，Jansen）领域的研究人员。主要用户将占仪器可用时间的约70%。由于我们预计IBL将持续增长，我们还为未成年人和新用户保留了30%的时间。 新系统将位于凯克自由电子激光中心，该中心位于范德比尔特校区的中心位置。这个多学科用户中心拥有广泛的共享光谱和成像系统，这些系统经常被来自生物科学，医学科学，物理科学和工程的众多研究人员使用。拟议系统的行政和管理将在细胞成像共享资源（ClSR）的保护伞下处理，该资源目前管理着10个共享显微镜和成像系统，其中两个与新仪器的位置相邻。