Q-TOF Mass Spectrometer for Peptide and Protein Analysis

描述（由申请人提供）：尽管普渡大学实力雄厚，但支持下一代生物学问题的分析仪器已经达到了极限。生物质谱是普渡大学的一个领域，在公认的研究优势（即仪器和应用开发）和为广泛的个人研究人员服务的能力之间存在二分法。该提案涉及扩展一个非常成功的校园质谱中心（CWMSC），以促进旨在使用质谱进行现代蛋白质分析的研究项目的发展。将开发一个新的CWMSC蛋白质分析核心来容纳拟议的仪器。多功能Q-TOF质谱仪被提议成为具有挑战性的蛋白质分析的核心，需要高灵敏度和质量分辨率。在普渡大学西拉斐特校区方圆60英里的范围内，没有类似的仪器为NIH的7个主要用户和8个次要用户提供服务。这些努力将补充以单个研究人员为基础的仪器及其应用开发研究，并为及时将蛋白质鉴定、定量和结构分析方面的最新方法转移到更广泛的生物学家群体提供平台。国内学术单位，普渡癌症中心和一个新的宾德利生物科学中心将合作，以确保适当的管理和蛋白质分析核心的必要扩展及时发生。大量的研究人员不仅需要使用该仪器，还需要聘请博士级别的科学家来支持该仪器和相关研究的额外专业知识。一些博士级的科学家和现有的研究小组也有可能参与生物质谱的开发和应用。

DESCRIPTION (provided by applicant): Despite the strengths at Purdue, the analytical instrumentation in support of the next generation of biological problems has been stretched to its limits. Biological mass spectrometry is an area at Purdue where a dichotomy exists between the recognized research strengths, that being instrumentation and application development, and the capacity to serve a broad range of individual investigators. The proposal involves the expansion of a highly successful Campus-Wide Mass Spectrometry Center (CWMSC) to foster the development of research programs that aim to conduct modern protein analyses using mass spectrometry. A new Protein Analysis Core of the CWMSC will be developed to house the proposed instrumentation. A versatile Q-TOF mass spectrometer is proposed to be the centerpiece for the challenging protein analysis requiring high sensitivity and mass resolution. There are no similar instruments to serve the NIH user group of 7 major and 8 minor users within a 60-mile radius of the Purdue West Lafayette campus. These efforts will complement the single investigator-based research in the development of instrumentation and their applications and provide a platform for timely transfer of state-of-art approaches in protein identification, quantitation and structural analysis to a broader group of biologists. The home academic units, the Purdue Cancer Center, and a new Bindley Bioscience Center will work cooperatively to ensure the proper management and needed expansion of the Protein Analysis Core occurs in a timely way. There are significant numbers of researchers that will need not only access to this instrument but also additional expertise of a Ph.D. level scientist who has been hired to support this instrumentation and the associated research. Several collaborative projects will also be possible for this Ph.D. level scientists and existing research groups that are involved in the development and applications of mass spectrometry in biology.