CCD Digital Imaging System for Core EM Facility

核心 EM 设施的 CCD 数字成像系统

• 批准号: 6731596
• 负责人: ROGER W CRAIG
• 金额: $ 19.85万
• 依托单位: UNIV OF MASSACHUSETTS MED SCH WORCESTER
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-04-01 至 2006-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6731596
• 关键词: bioimaging /biomedical imaging; biomedical equipment purchase; charge coupled device camera; cryoelectron microscopy; digital imaging; transmission electron microscopy

DESCRIPTION (provided by applicant): This application is a request for funds to provide CCD digital imaging capability for 11 major and 5 minor users of TEMs in the Core Electron Microscopy Facility of the University of Massachusetts Medical School (UMMS). Ultrastructural projects range from the tissue to the molecular level, and include low dose, tomographic, cryo-EM, serial section, immunolabeling, and morphological studies of a wide range of biomedically important systems. The major benefit of direct digital image acquisition is the instantaneous feedback that it provides, in the form of immediate images and instant knowledge of imaging parameters and specimen characteristics. These benefits make a critical difference to specialist applications, such as cryo-EM and tomography, where they can save hundreds of hours of work. In some cases studies become possible for the first time using CCD technology. The benefits of CCD imaging are also key to enhancing productivity in more routine applications. Digital acquisition not only adds convenience and saves time (by eliminating the film processing, printing, and scanning necessary for analysis and publication of images), but also results in major cost savings. In the past year, UMMS researchers spent approximately $40,000 in supplies and personnel costs for developing, printing and scanning the approximately 10,000 micrographs that were taken, all on film. Digital imaging would have eliminated almost all of these costs. Finally, digital imaging is important in teaching, facilitating instruction of novice microscopists and providing stimulating images for visiting medical, graduate and high school students. To provide for the needs of both specialist and routine TEM users alike, we are requesting funds to acquire digital imaging capability for two microscopes. These microscopes perform complementary tasks: one is reserved specifically for specialist applications, including low dose microscopy, cryo-EM and tomography, while the other is used for routine microscopy. This division of labor avoids any compromise to the functioning of the specialist microscope, where optimal optical, vacuum and mechanical performance are crucial. Equipping both microscopes with slow scan CCD cameras will extend the advantages of this technology to all researchers who require it. A third TEM will continue to operate with film for researchers who prefer this medium.

描述(由申请人提供)：本申请是一项资金申请，为马萨诸塞大学医学院(UMMS)核心电子显微镜设备的11名主要用户和5名次要用户提供CCD数字成像能力。超微结构项目从组织到分子水平，包括低剂量、断层扫描、冷冻-EM、连续切片、免疫标记和对广泛的生物医学重要系统的形态研究。 直接数字图像采集的主要好处是它以即时图像和成像参数和标本特征的即时知识的形式提供即时反馈。这些好处对冷冻-EM和断层扫描等专业应用产生了至关重要的影响，在这些应用中，它们可以节省数百个小时的工作。在某些情况下，首次使用电荷耦合器件技术进行研究成为可能。在更常规的应用中，CCD成像的好处也是提高生产率的关键。数字采集不仅增加了便利性并节省了时间(通过消除分析和发布图像所需的胶片处理、打印和扫描)，还节省了大量成本。在过去的一年里，密歇根大学的研究人员花费了大约40,000美元的用品和人员成本来冲洗、打印和扫描大约10,000张显微照片，所有这些照片都是在胶片上拍摄的。数字成像本可以消除几乎所有这些成本。最后，数字成像在教学中很重要，有助于指导显微镜新手，并为来访的医学、研究生和高中生提供刺激的图像。 为了满足专业和常规的透射电子显微镜用户的需求，我们正在申请资金，以获得两台显微镜的数字成像能力。这些显微镜执行互补的任务：一台专门为专业应用保留，包括低剂量显微镜、冷冻-EM和断层扫描，而另一台用于常规显微镜。这种分工避免了对专业显微镜功能的任何妥协，在专业显微镜中，最佳的光学、真空和机械性能至关重要。为这两台显微镜配备慢扫描CCD摄像头，将把这项技术的优势扩展到所有需要它的研究人员身上。第三个瞬变显微镜将继续与胶片一起工作，供喜欢这种介质的研究人员使用。