Scanning Electron Microscope for Core EM Facility

核心 EM 设施的扫描电子显微镜

• 批准号: 7212260
• 负责人: ROGER W CRAIG
• 金额: $ 49.82万
• 依托单位: UNIV OF MASSACHUSETTS MED SCH WORCESTER
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-01-15 至 2008-01-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7212260
• 关键词: 15 year old; Age; Area; Arts; Bacterial Infections; Cell Surface Receptors; Cell surface; Cells; Cellular Structures; Cereals; Cilia; Data Collection; Detection; Development; Disease; Drosophila genus; Electron Microscope; Electron Microscopy Facility; Funding; Grant; Guns; Housing; Image; Immune; Infection; Ions; Knowledge; Massachusetts; Microscope; Minor; Modeling; Mus; Play; Proteins; Purpose; Research Personnel; Resolution; Role; Scanning; Scanning Electron Microscopy; Services; Signal Transduction; Speed; Structure; Surface Antigens; Time; Universities; bone; human disease; improved; insight; instrument; medical schools; mutant; quantum; repaired

DESCRIPTION (provided by applicant): We request funds for the purchase of an FEI Quanta 200 field emission scanning electron microscope (FESEM) together with a Gatan Model 681 ion beam sputter coater. The microscope and sputter coater will provide for the scanning electron microscopy needs of nine major and three minor users at the University of Massachusetts Medical School. The microscope will be housed in the Core Electron Microscopy Facility of the Medical School, and will replace the only other instrument available - an obsolete 30-year old SEM that is out of service much of the time, lacks key capabilities, and is proving impossible to repair due to its age and the unavailability of spare parts. The ion beam sputter coater will replace a conventional 15-year old sputter coater and is required to provide the fine grain coatings needed for high resolution studies. The projects that will use SEM include studies of the functioning of cell surface receptors involved in immune recognition, the development and function of cilia, the structure of normal and diseased bone, structural changes in mutants of signaling and other proteins in mice and Drosophila, and cell surface structures involved in bacterial infection. These projects require FESEM for two main purposes - the imaging of cell surface structure at high resolution, and the detection and localization of specific cell surface antigens by immuno-FESEM. These images will provide essential insights into the mechanisms of cell development, function, infection, and disease. In addition to the users on this grant, there are approximately twenty other investigators at the medical school who would use SEM if we had a functioning instrument. A state-of-the-art SEM, with field emission gun, has become essential to meet this need. It will transform the ability of investigators to carry out their SEM-related studies, it will greatly improve the quality and speed of data collection, and it will make possible observations that are impossible on our current instrument. All of the projects that will use this microscope aim to provide fundamental insights into cellular structure and function. Many have a direct bearing on the understanding of human disease. Results provided by this instrument will play a key role in advancing our knowledge in these areas.

描述（由申请人提供）：我们要求购买FEI Quanta 200场发射扫描电子显微镜（FESEM）以及GATAN型号681离子束溅射座椅夹。显微镜和溅射夹将在马萨诸塞大学医学院的九个主要和三个未成年人使用的扫描电子显微镜需求提供。显微镜将安置在医学院的核心电子显微镜设施中，并将替换唯一可用的其他仪器 - 在大部分时间无法使用的30岁过时的SEM，缺乏关键功能，并且由于其年龄和零件的不可行性而无法修复。离子束溅射夹将取代常规的15岁溅射夹具，并需要提供高分辨率研究所需的细粒涂层。将使用SEM的项目包括对涉及免疫识别的细胞表面受体的功能，纤毛的发育和功能，正常骨和患病的结构，小鼠和果蝇中信号传导和其他蛋白质突变体的结构变化，以及涉及细菌感染的细胞表面结构。这些项目需要FESEM出于两个主要目的 - 高分辨率以细胞表面结构的成像，以及通过免疫FESEM检测和定位特定细胞表面抗原。这些图像将提供有关细胞发育，功能，感染和疾病机制的重要见解。除了这笔赠款的用户外，医学院还有大约二十名其他调查人员，如果我们有运行的工具，他们将使用SEM。具有现场排放枪的最先进的SEM对满足这一需求至关重要。它将改变研究人员进行与SEM相关的研究的能力，它将大大提高数据收集的质量和速度，并且它将使我们当前的仪器无法观察到可能的观察结果。所有将使用此显微镜的项目旨在提供对细胞结构和功能的基本见解。许多人直接与对人类疾病的理解有关。该工具提供的结果将在促进我们在这些领域的知识中发挥关键作用。

项目成果

An amphiphilic degradable polymer/hydroxyapatite composite with enhanced handling characteristics promotes osteogenic gene expression in bone marrow stromal cells.

• DOI: 10.1016/j.actbio.2013.06.013
• 发表时间: 2013-09
• 期刊: ACTA BIOMATERIALIA
• 影响因子: 9.7
• 作者: Kutikov, Artem B.;Song, Jie
• 通讯作者: Song, Jie

Rapid prototyping amphiphilic polymer/hydroxyapatite composite scaffolds with hydration-induced self-fixation behavior.

• DOI: 10.1089/ten.tec.2014.0213
• 发表时间: 2015-03
• 期刊: Tissue engineering. Part C, Methods
• 作者: A. Kutikov;A. Gurijala;Jie Song

The lncRNA HOXA11os regulates mitochondrial function in myeloid cells to maintain intestinal homeostasis.

lncRNA HOXA11os 调节骨髓细胞中的线粒体功能以维持肠道稳态。

• DOI: 10.1016/j.cmet.2023.06.019
• 发表时间: 2023
• 期刊: Cell metabolism
• 影响因子: 29
• 作者: Shmuel-Galia,Liraz;Humphries,Fiachra;Vierbuchen,Tim;Jiang,Zhaozhao;Santos,Nolan;Johnson,John;Shklyar,Boris;Joannas,Leonel;Mustone,Nicholas;Sherman,Shany;Ward,Doyle;Houghton,JeanMarie;Baer,ChristinaE;O'Hara,Aisling;Henao-Mejia,
• 通讯作者: Henao-Mejia,

Structural properties of optically clear bacterial cellulose produced by Komagataeibacter hansenii using arabitol.

• DOI: 10.1016/j.bioadv.2023.213345
• 发表时间: 2023-02
• 期刊: Biomaterials advances
• 作者: Elizabeth M. van Zyl;M. Kennedy;Wendy Nason;Sawyer J. Fenlon;E. Young;Luis J. Smith;S. Bhatia;J. Coburn

Sustained and localized in vitro release of BMP-2/7, RANKL, and tetracycline from FlexBone, an elastomeric osteoconductive bone substitute.

• DOI: 10.1002/jor.20890
• 发表时间: 2009-10
• 期刊: JOURNAL OF ORTHOPAEDIC RESEARCH
• 影响因子: 2.8
• 作者: Xu, Jianwen;Li, Xinning;Lian, Jane B.;Ayers, David C.;Song, Jie
• 通讯作者: Song, Jie