Multi-Parameter Bench-top Flow Cytometer

描述（由申请人提供）：流式细胞术是生物医学研究中用于细胞和亚细胞分析的强大技术。其主要功能在于能够快速对单个细胞进行多参数分析，从而能够识别和分离重要的细胞亚群。位于查佩尔山的北卡罗来纳州大学的流式细胞仪设施成立于1982年，以提供这一关键的研究核心服务。该设施是向联合国大学日内瓦分校研究人员提供研究流式细胞仪服务的唯一机构，目前有3台分析流式细胞仪（2台FACScans、1台FACSCalibur）和1台分选机（MoFIo）。这些仪器的使用率很高（上一财政年度收费5 174.5小时），每年为100多名调查员服务。在本财政年度的前6个月（2002年7月1日至2002年12月31日），向81名调查员收取了2 699个小时的费用。我们机构在传染病研究方面的一项新的重大举措促使我们要求将现有的FACSCalibur转移到BSL-3设施，为传染病研究人员提供对活的传染性样本进行流式细胞术的能力。这将严重干扰我们的机器访问时间为一般用途，并删除我们的能力，做四色分析所需要的一个越来越多的研究小组。因此，我们必须在允许这一举动之前更换FACSCalibur。此外，一些研究人员要求增加参数数量（5+颜色），更多和更低成本的Ca++动员实验，CFP的激发，以及使用Hoechst或DAPI的DNA含量分析。因此，为了满足这些不断增长的需求，更换FACSCalibur的4色能力和一般能力，并为未来的发展做好准备，我们正在申请资金购买一台四激光多参数台式分析流式细胞仪。目前，我们认为DakoCytomation的CyAn最能满足我们的要求，并在此申请中要求大部分资金用于购买仪器。北卡罗来纳州医学院提供65,500美元用于购买，除了所有安装/修改费用（9,500美元）。该学院还提供了相当大的持续支持对流式细胞仪设施的操作-$80，000/年从Uneberger综合癌症中心和25%的设施主任的工资从微生物学和免疫学系。

DESCRIPTION (provided by applicant): Flow cytometry is a powerful technology for cellular and subcellular analyses in biomedical research. Its primary power lies in its ability to rapidly perform multi-parametric analysis of individual cells, thereby permitting the identification and isolation of important subsets of cells. The Flow Cytometry Facility at The University of North Carolina at Chapel Hill was established in 1982 to provide this critical research core service. The Facility is the sole provider of research flow cytometry services to investigators at UNC-CH. The Facility currently has 3 analytical flow cytometers (2 FACScans, 1 FACSCalibur) and one sorter (MoFIo). The instruments are heavily used (last fiscal year 5,174.5 hours charged) and annually serve over 100 investigators. During the first 6 months of this fiscal year (July 1,2002 to Dec. 31, 2002) 2,699 hours have been charged to 81 investigators. A new major initiative in infectious diseases research at our institution has prompted a request that we move the existing FACSCalibur to a BSL-3 facility to provide infectious disease investigators the ability to perform flow cytometry on viable infectious samples. This would seriously interfere with our machine access time for general use and remove our ability to do 4-color analysis required by a growing group of investigators. Thus, we must replace the FACSCalibur before permitting this move. In addition, several investigators are requiring increased number of parameters (5+ colors), more and lower cost Ca++ mobilization experiments, excitation of CFP, and DNA content analysis using Hoechst or DAPI. Thus, in order to meet these increasing demands, replace the FACSCalibur's 4-color capability and general capacity, and provide for future developments we are requesting funds to purchase a four-laser, multi-parameter bench top analytical flow cytometer. At present, we believe the CyAn from DakoCytomation best fulfills our requirements and are requesting, in this application, the majority of funds to purchase the instrument. The University of North Carolina School of Medicine is providing $65,500 towards the purchase in addition to all installation/modification costs ($9,500). The School is also providing considerable on-going support towards the operation of The Flow Cytometry Facility - $80,000/year from The Uneberger Comprehensive Cancer Center and 25% of the Facility Director's salary from The Department of Microbiology and Immunology.