FLOW CYTOMETRY SORTER FOR UNIV OF NC AT CHAPEL HILL: IMMUNOLOGY

该子项目是利用由NIH/NCRR资助的共享仪器补助金提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是资助机构，不一定是研究者的机构。描述（由申请人提供）：我们正在申请资金购买DakoCytomation MoFlo分选机。北卡罗来纳大学教堂山分校流式细胞术设施是我们大型研究机构中唯一提供流式细胞术服务的机构，该设备需要增加日益繁忙的分选能力。该设施目前有5台流式细胞仪（2台facscan， 1台FACSCalibur， 2台CyAns）和1台分选仪。第二台MoFlo分选机（本申请中描述的仪器）将于2005年3月底交付，由于非常需要，该大学已同意租赁（无购买选择权）该仪器，以便在获得购买该仪器的资金之前提供迫切需要的能力。该设施正在支持越来越多的nih资助的研究人员，他们需要更多地获得这项关键的研究技术。在2004年度，该设施提供了7,351小时的收费服务，其中1,170小时是分类服务。我们的服务支持16个不同部门和11个研究中心的140多个实验室（其中60个在CY2004中使用）的研究，其中至少有5个是NIH指定的研究中心。所要求的仪器的能力将支持所有分选机用户的研究，但我们在本申请中选择了21名研究人员，作为正在进行的研究类型的代表。总的来说，预计在未来一年，所有用户将需要这两种仪器80%以上的容量。我们选择MoFlo不仅是因为我们发现它是一种非常可靠，优秀的仪器，还因为我们强烈认为，拥有2台相同的分选机在培训操作员，仪器之间的操作员交换，易于故障排除以及公司沟通和服务方面为设施提供了强大的优势。所要求的仪器将提供3个激光激发，使用所有二极管/固态激光器，自动克隆，排序室的气溶胶排出，数字电子，4路分选，11个荧光探测器和样品温度控制。我们认为这一文书与公共卫生密切相关。及时进行高质量的基础和转化生物医学研究，对于增进对疾病的了解和制定基于知识的合理干预策略至关重要。流式细胞术是生物医学研究人员的一个强大工具，可以帮助他们实现生物医学研究目标。

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): We are requesting funds to purchase a DakoCytomation MoFlo sorter. This instrument is needed to augment the sorting capacity of the increasingly busy University of North Carolina at Chapel Hill Flow Cytometry Facility, which is the only provider of research flow cytometry services at our large research institution. The Facility currently has 5 analytical flow cytometers (2 FACScans, 1 FACSCalibur, 2 CyAns) and one sorter. A second MoFlo sorter (the instrument described in this application), to be delivered in late March 2005, is so much needed that the University has agreed to lease (no purchase option) the instrument in order to provide the desperately needed capacity until funding can be secured to purchase the instrument. The Facility is supporting an ever-larger group of NIH-funded investigators needing increasing access to this key research technology. In CY2004 the Facility performed 7,351 hours of charged service of which 1,170 hours was for sorting. Our services support the research of over 140 laboratories (60 used sorting in CY2004) in 16 different departments and 11 research centers, at least 5 of which are designated NIH research centers. The capacity of the requested instrument will support the research of all sorter users but we have selected 21 investigators to highlight in this application as being representative of the types of research being performed. In total, all users are expected to need more than 80% of the capacity of both instruments in the upcoming year. We have chosen the MoFlo not only because we have found it to be a highly dependable, excellent instrument but also because we feel strongly that having 2 identical sorters provides strong advantages for the Facility in terms of training operators, interchange of operators between instruments, ease of troubleshooting and company communication and service. The instrument requested will provide 3 laser excitation using all diode/solid-state lasers, automated cloning, aerosol evacuation of sort chamber, digital electronics, 4-way sorting, 11 fluorescence detectors, and sample temperature control. We feel this instrument is very relevant to public health. Timely high quality basic and translational biomedical research is fundamental to the development of an understanding of disease and to the development of knowledge-based rational intervention strategies. Flow cytometry is a powerful tool in the arsenal of the biomedical researcher and, thus helps them realize their biomedical research goals.