FLOW CYTOMETRY SORTER FOR UNIV OF NC AT CHAPEL HILL: BIOCHEMISTRY

北卡罗来纳大学教堂山分校的流式细胞术分选机：生物化学

• 批准号: 7335079
• 负责人: LARRY W. ARNOLD
• 金额: $ 6.13万
• 依托单位: UNIV OF NORTH CAROLINA CHAPEL HILL
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-04-01 至 2007-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7335079
• 关键词: aerosols; biochemistry; communication; flow cytometry; fluorescence; lasers; public health; solid state; temperature; training; university

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): We are requesting funds to purchase a DakoCytomation MoFlo sorter. This instrument is needed to augment the sorting capacity of the increasingly busy University of North Carolina at Chapel Hill Flow Cytometry Facility, which is the only provider of research flow cytometry services at our large research institution. The Facility currently has 5 analytical flow cytometers (2 FACScans, 1 FACSCalibur, 2 CyAns) and one sorter. A second MoFlo sorter (the instrument described in this application), to be delivered in late March 2005, is so much needed that the University has agreed to lease (no purchase option) the instrument in order to provide the desperately needed capacity until funding can be secured to purchase the instrument. The Facility is supporting an ever-larger group of NIH-funded investigators needing increasing access to this key research technology. In CY2004 the Facility performed 7,351 hours of charged service of which 1,170 hours was for sorting. Our services support the research of over 140 laboratories (60 used sorting in CY2004) in 16 different departments and 11 research centers, at least 5 of which are designated NIH research centers. The capacity of the requested instrument will support the research of all sorter users but we have selected 21 investigators to highlight in this application as being representative of the types of research being performed. In total, all users are expected to need more than 80% of the capacity of both instruments in the upcoming year. We have chosen the MoFlo not only because we have found it to be a highly dependable, excellent instrument but also because we feel strongly that having 2 identical sorters provides strong advantages for the Facility in terms of training operators, interchange of operators between instruments, ease of troubleshooting and company communication and service. The instrument requested will provide 3 laser excitation using all diode/solid-state lasers, automated cloning, aerosol evacuation of sort chamber, digital electronics, 4-way sorting, 11 fluorescence detectors, and sample temperature control. We feel this instrument is very relevant to public health. Timely high quality basic and translational biomedical research is fundamental to the development of an understanding of disease and to the development of knowledge-based rational intervention strategies. Flow cytometry is a powerful tool in the arsenal of the biomedical researcher and, thus helps them realize their biomedical research goals.

该子项目是利用由NIH/NCRR资助的共享仪器赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。列出的机构是用于资助的，不一定是研究者的机构。描述（由申请人提供）：我们正在申请资金以购买DakoCytomation MoFlo分选机。这台仪器是需要增加日益繁忙的忙碌北卡罗来纳州大学查佩尔山流式细胞仪设施的分选能力，这是我们大型研究机构中研究流式细胞仪服务的唯一提供者。该设施目前有5台分析流式细胞仪（2台FACScans、1台FACSCalibur、2台CyAns）和1台分选机。第二个MoFlo分选机（本申请中所述的仪器），将于2005年3月下旬交付，是如此的需要，该大学已同意租赁（没有购买选项）的仪器，以提供迫切需要的能力，直到资金可以得到保证购买仪器。该基金正在支持越来越多的NIH资助的研究人员，他们需要更多地获得这一关键研究技术。在2004年，该设施提供了7，351小时的收费服务，其中1，170小时用于分拣。我们的服务支持16个不同部门和11个研究中心的140多个实验室（2004年有60个实验室使用了分类）的研究，其中至少有5个是指定的NIH研究中心。所需仪器的容量将支持所有分拣机用户的研究，但我们选择了21名研究人员在本申请中突出显示，作为正在进行的研究类型的代表。总体而言，预计所有用户在未来一年将需要这两种仪器80%以上的容量。我们选择MoFlo不仅是因为我们发现它是一种高度可靠的优秀仪器，还因为我们强烈认为拥有2台相同的分拣机在培训操作员、仪器之间的操作员交换、故障排除的方便性以及公司沟通和服务方面为工厂提供了强大的优势。所要求的仪器将提供3个激光激发（使用所有二极管/固态激光器）、自动克隆、分选室气溶胶排空、数字电子设备、4路分选、11个荧光检测器和样品温度控制。我们认为这一文书与公共卫生非常相关。及时开展高质量的基础和转化生物医学研究，对于发展对疾病的认识和制定以知识为基础的合理干预战略至关重要。流式细胞术是生物医学研究人员武器库中的强大工具，从而帮助他们实现其生物医学研究目标。