Acquisition of a 700MHz NMR Spectrometer and Cryoprobe

该提案要求提供部分资金，用于购买一台配备低温冷却探头的700兆赫核磁共振波谱仪。核磁共振仪器将支持健康相关科学的研究工作，并通过NIH赞助的研究和机构培训赠款为本科生、研究生和研究生研究员提供培训。获得这种最先进的仪器对于北卡罗来纳州大学的NIH研究人员在以下领域进行的生物医学研究至关重要：1）GTTRac-1的结构研究，坎贝尔小组; 2）DBL蛋白的结构和调节，坎贝尔小组; 3）蛋白质中运动偶联效应的2 H弛豫研究，A.李氏集团; 4）结构 结合组蛋白mRNA的3'末端的蛋白质的研究，Marzluff组; 5）来源于粘着斑激酶（FAK）的FAT结构域的结构和动力学分析，Szuer组; 6）FAT-桩蛋白复合物的结构研究，Szuer组; 7）铂加合物的易错复制旁路，Chaney组; 8）活大肠杆菌细胞中蛋白质的NMR研究，Pielak组; 9）计算机设计的蛋白质的结构分析，Kuhlman组; 10）生物活性天然产物及其衍生物的鉴定和结构表征，K.H.李氏集团; 11）代谢组学，MacDonald小组; 12）DNA损伤和修复，Swenberg小组; 13）肽二级结构中稳定性和特异性的贡献因子， 沃茨组; 14）胞苷脱氨酶的结构研究，沃尔芬登。NMR仪器将被安置在查佩尔山的北卡罗来纳州大学的生物分子NMR设施（UNC-CH）中。所要求的仪器对于支持和开发 在北卡罗来纳州大学的研究计划，以及加强和扩大学生在化学，生物物理学和生物化学的教育。所要求的仪器也将提供给在联合国大学-瑞士中心以外从事生物医学研究的其他研究人员使用。

This proposal requests partial funding for the purchase of a 700 MHz NMR spectrometer with a cryogenically cooled probe. The NMR instrumentation will support research efforts in the health-allied sciences and provide training of undergraduate, graduate, and postgraduate fellows through NIH-sponsored research and institutional training grants. Acquisition of this state-of-the-art instrumentation is essential for biomedical research being performed by NIH researchers at the University of North Carolina in the following areas: 1) Structural studies of the GTPase Rac-1, Campbell group; 2) Structure and regulation of DBL proteins, Campbell group; 3) 2H relaxation studies of motional coupling effects in proteins, A. Lee group; 4) Structural studies of proteins that bind the 3' end of histone mRNA, Marzluff group; 5) Structural and dynamic analysis of the FAT domain derived from Focal Adhesion Kinase (FAK), Schaller group; 6) Structural studies of the FAT-Paxillin complex, Schaller group; 7) Error-prone replicative bypass of platinum adducts, Chaney group; 8) NMR studies of proteins in living Escherichia Coli cells, Pielak group; 9) Structural analysis of computer-designed proteins, Kuhlman group; 10) Identification and structural characterization of bioactive natural products and their derivatives, K.H. Lee group; 11) Metabolomics, MacDonald group; 12) DNA damage and repair, Swenberg group; 13) Contributing factors to stability and specificity in peptide secondary structure, Waters group; 14) Structural studies of cytidine deaminase, Wolfenden. The NMR instrumentation will be housed in the UNC Biomolecular NMR facility at the University of North Carolina at Chapel Hill (UNC-CH). The instrumentation requested is critical for the support and development of research programs at the University of North Carolina, as well as to enhance and expand the education of students in chemistry, biophysics, and biochemistry. The requested instrument will also be available for use by other researchers outside of UNC-CH pursuing biomedical research.