Leica TCS SP2 AOBS Vis-UV Confocal Microscope

Leica TCS SP2 AOBS 可见光-紫外共焦显微镜

• 批准号: 6580059
• 负责人: MARGARET T FULLER
• 金额: $ 42.5万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-05-01 至 2004-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6580059
• 关键词: biomedical equipment purchase; confocal scanning microscopy; protein localization; protein protein interaction

DESCRIPTION (provided by applicant): This proposal requests support for the purchase of a Leica TCS SP2 AOBS Vis-UV Confocal Microscope. This microscope, which will be a shared resource, will be crucial for the ongoing research of investigators working in several departments at the Stanford University School of Medicine. The projects of the major users investigate a range of NIH supported topics, including: i) cell polarity and asymmetric cell division in bacteria (Shapiro), epithelial cells (Nelson) and stem cells (Fuller), ii) intercellular signaling and cell fate in bacteria (Kaiser), mouse embryonic and neural stem cells (Nusse), and gametogenesis (Tazuke), iii) mechanisms of localized intracellular signaling by small molecule second messengers (Conti), iv) transcriptional regulation of neuronal and cardiovascular development (Crabtree), v) mechanisms of cytoskeletal function and membrane trafficking during cell division (Fuller), and vi) mechanisms of chromosome pairing and synapsis during meiosis (Villeneuve). All of these projects require precise localization of proteins or other cellular components in cultured cells or complex tissues. The ability to precisely localize proteins to specific cellular compartments and to infer protein interactions by colocalization provided by the requested shared Laser Scanning Confocal microscope will be crucial to elucidating the normal and pathogenetic mechanisms that regulate and mediate the important developmental and cellular events under investigation. The above projects are being undertaken by senior, intermediate, and recently recruited faculty members, who will cooperate in supporting this confocal facility. The enhanced resolution and sensitivity of this microscope, as well as its ability to effectively image several different fluors simultaneously in both single cells and in thicker tissue, will be crucial to the success of these NIH funded projects.

描述（由申请人提供）： 该建议要求购买Leica TCS SP2 AOBS VIS-UV共聚焦显微镜。该显微镜将是一种共享资源，对于在斯坦福大学医学院多个部门工作的研究人员正在进行的研究至关重要。主要用户的项目研究了一系列NIH支持的主题，包括：i）细菌（Shapiro）（Shapiro）（Shapiro），上皮细胞（Nelson）和干细胞（FULLER）的细胞极性和不对称细胞分裂，II）细菌（ii）细菌（KAISER）（KAISER），胚胎和神经细胞（NUSSE）（NUSSE）（NUSSE）（NUSSE）（NUSSEN）（TAZ）的细胞间信号传导和细胞命运（TAZ）小分子的细胞内信号第二信使（conti），iv）神经元和心血管发育的转录调控（crabtree），v）细胞骨架功能和细胞分裂期间（富勒）和VI期间细胞骨架功能和膜运输机制的机制。所有这些项目都需要在培养的细胞或复杂组织中精确地定位蛋白质或其他细胞成分。精确将蛋白质定位于特定细胞室的能力以及通过请求的共享激光扫描共聚焦显微镜提供的共定位来推断蛋白质相互作用，对于阐明调节和调节重要的发育和细胞的正常和致病机制至关重要。上述项目是由高级，中级和最近招募的教职员工进行的，他们将合作支持这一共识设施。该显微镜的分辨率和灵敏度的增强，及其在单个细胞和较厚组织中同时有效成像几种荧光的能力对这些NIH资助的项目的成功至关重要。