Leica TCS SP2 AOBS Vis-UV Confocal Microscope

描述(由申请人提供)： 该提案请求支持购买徕卡TCS SP2 AOBS VIS-UV共聚焦显微镜。这种显微镜将成为共享资源，对斯坦福大学医学院几个部门的研究人员正在进行的研究至关重要。主要用户的项目研究NIH支持的一系列主题，包括：i)细菌(Shapiro)、上皮细胞(Nelson)和干细胞(Fuller)中的细胞极性和不对称细胞分裂；ii)细菌(Kaiser)、小鼠胚胎和神经干细胞(Nusse)中的细胞间信号和细胞命运以及配子发生(Tazuke)；iii)小分子第二信使(CONTI)定位的细胞内信号转导机制；iv)神经元和心血管发育的转录调控(Crabtree)；v)细胞分裂中细胞骨架的功能和膜的运输(Fuller)机制；以及)染色体配对和联会在减数分裂过程中的机制(Villenve)。所有这些项目都需要对培养细胞或复杂组织中的蛋白质或其他细胞成分进行精确定位。由所要求的共享激光扫描共聚焦显微镜提供的精确定位特定细胞间隔的蛋白质并通过共定位推断蛋白质相互作用的能力对于阐明调节和调节正在研究的重要发育和细胞事件的正常和致病机制将是至关重要的。上述项目由高级、中级和新近招聘的教职员工承担，他们将合作支持这一共焦设施。这种显微镜的分辨率和灵敏度的提高，以及它在单个细胞和更厚的组织中同时有效地成像几种不同荧光的能力，将是NIH资助的这些项目成功的关键。

DESCRIPTION (provided by applicant): This proposal requests support for the purchase of a Leica TCS SP2 AOBS Vis-UV Confocal Microscope. This microscope, which will be a shared resource, will be crucial for the ongoing research of investigators working in several departments at the Stanford University School of Medicine. The projects of the major users investigate a range of NIH supported topics, including: i) cell polarity and asymmetric cell division in bacteria (Shapiro), epithelial cells (Nelson) and stem cells (Fuller), ii) intercellular signaling and cell fate in bacteria (Kaiser), mouse embryonic and neural stem cells (Nusse), and gametogenesis (Tazuke), iii) mechanisms of localized intracellular signaling by small molecule second messengers (Conti), iv) transcriptional regulation of neuronal and cardiovascular development (Crabtree), v) mechanisms of cytoskeletal function and membrane trafficking during cell division (Fuller), and vi) mechanisms of chromosome pairing and synapsis during meiosis (Villeneuve). All of these projects require precise localization of proteins or other cellular components in cultured cells or complex tissues. The ability to precisely localize proteins to specific cellular compartments and to infer protein interactions by colocalization provided by the requested shared Laser Scanning Confocal microscope will be crucial to elucidating the normal and pathogenetic mechanisms that regulate and mediate the important developmental and cellular events under investigation. The above projects are being undertaken by senior, intermediate, and recently recruited faculty members, who will cooperate in supporting this confocal facility. The enhanced resolution and sensitivity of this microscope, as well as its ability to effectively image several different fluors simultaneously in both single cells and in thicker tissue, will be crucial to the success of these NIH funded projects.