DNA Sequencer for Shared Resource Lab

共享资源实验室 DNA 测序仪

• 批准号: 6580935
• 负责人: MARK O. LIVELY
• 金额: $ 14.36万
• 依托单位: WAKE FOREST UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-05-01 至 2004-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6580935
• 关键词: biomedical equipment purchase; nucleic acid quantitation /detection; nucleic acid sequence

DESCRIPTION (provided by applicant): This grant application requests funding for the purchase of an Applied Biosystems 3100 Genetic Analyzer to replace a six year old Applied Biosystems 377 DNA Sequencer that is operated by the DNA Sequencing Core lab of Wake Forest University School of Medicine (WFUSM). The existing DNA sequencer provides support for over 45 funded NIH grants. Over 90% of the samples submitted by WFUSM faculty are funded by NIH grant money. The DNA sequencing laboratory offers low cost, accurate sequencing technology to investigators in a convenient, on-campus setting. The demand for automated DNA sequencing as a critical research tool has grown steadily, and with the addition of up to ten faculty positions in 2002, an increased demand in services of about 25% over the next two years is anticipated. While the current instrumentation has performed well, slab gel technology is rapidly being replaced by more sensitive and efficient capillary electrophoresis. The decision by Applied Biosystems, the major supplier of sequencing technology, to discontinue the 377 DNA sequencer guarantees that the 377 DNA sequencer currently in use will become obsolete. Replacement of the 377 with the 3100 Genetic Analyzer will ensure that researchers have continued access to the most up-to-date, sensitive sequencing technology, and will allow the laboratory to provide rapid delivery of sequencing data to investigators. The proposed instrumentation will be available to the entire faculty on a fee-for-service basis. The lab has been operated in this way for six years, under the direction of Dr. Mark Lively and the supervision of Ms. Elyse Jung. While most of the support for the instrumentation will come from user fees, additional support will be provided by the Comprehensive Cancer Center of Wake Forest University School of Medicine. The combination of high quality instrumentation, experienced personnel, and solid financial support will ensure that NIH-funded researchers will benefit from acquisition of the proposed instrumentation.

描述（由申请人提供）：此资助申请要求资助购买应用生物系统3100遗传分析仪，以取代由维克森林大学医学院（WFUSM）DNA测序核心实验室操作的六年之久的应用生物系统377 DNA测序仪。现有的DNA测序仪为超过45个NIH资助赠款提供支持。WFUSM教师提交的样本中有90%以上是由NIH资助的。DNA测序实验室提供低成本，准确的测序技术，在一个方便的，校园内的设置调查。自动化DNA测序作为一种重要的研究工具的需求稳步增长，随着2002年增加多达10个教师职位，预计未来两年对服务的需求将增加约25%。虽然目前的仪器表现良好，但平板凝胶技术正迅速被更灵敏、更有效的毛细管电泳所取代。测序技术的主要供应商应用生物系统公司决定停止使用377 DNA测序仪，这保证了目前使用的377 DNA测序仪将被淘汰。用3100基因分析仪取代377基因分析仪将确保研究人员继续获得最新、最灵敏的测序技术，并使实验室能够向研究人员快速提供测序数据。拟议的仪器将提供给整个教师的收费服务的基础上。在Mark Lively博士的指导和Elyse Jung女士的监督下，该实验室以这种方式运作了六年。虽然仪器的大部分支持将来自用户费用，但维克森林大学医学院综合癌症中心将提供额外的支持。高质量的仪器，经验丰富的人员和坚实的财政支持相结合，将确保NIH资助的研究人员将受益于收购拟议的仪器。