Biomolecular Resource Laboratory

生物分子资源实验室

• 批准号: 7529742
• 负责人: MARK O. LIVELY
• 金额: $ 8.17万
• 依托单位: WAKE FOREST UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-07-11 至 2012-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7529742
• 关键词: Accounting; Area; Arts; Biotechnology; Cancer Center; Chemicals; Clinical Research; Comprehensive Cancer Center; Comprehensive Cancer Center of Wake Forest University; Consult; Consultations; Custom; DNA; DNA Damage; DNA Sequence; DNA Sequence Analysis; DNA laboratory; Data; Data Analyses; Doctor of Philosophy; Equipment; Experimental Designs; Funding; Grant; Gray unit of radiation dose; Internet; Ions; Laboratories; Laboratory Research; Lipids; Location; Malignant Neoplasms; Mass Spectrum Analysis; Metabolic; Oligonucleotides; Peptide Synthesis; Peptides; Play; Protein Analysis; Protein Biochemistry; Proteins; Proteomics; RNA chemical synthesis; Reporting; Research; Research Personnel; Resources; Role; Sampling; Services; Support of Research; Technology; Time; Triad Acrylic Resin; United States National Institutes of Health; Water; analytical method; anticancer research; cell growth; cost; data acquisition; design; experience; instrumentation; mass spectrometer; member; metabolomics; novel; peptide chemical synthesis; programs; research study

The Biomolecular Resource Laboratory (BRL) is a heavily used core service laboratory of the Comprehensive Cancer Center that supported the research laboratory activities of 41 Cancer Center members during fiscal year 2005. The BRL provides strategically important analytical services to Center members for 1) characterization of DMA, peptides, proteins and 2) identification and quantification of lipids, proteins, and peptides. These services include five different types of mass spectrometry for analysis of proteins, peptides and lipids, automated DNA sequence analysis, synthesis of RNA and DNA oligonucleotides, purification of peptides and proteins, and chemical synthesis of peptides. The BRL analyzed more than 14,000 samples during the reporting period. DNA sequence analysis (7924 samples) and mass spectrometry (6403 samples) were the most heavily used core services in the BRL. The protein/peptide and DNA chemical services accounted for an additional 956 samples. Laboratories of Cancer Center members received 49% of the services performed in the BRL during fiscal year 2005. The BRL has expanded significantly since the last review. Mass spectrometry services have been added to meet the significant needs of Cancer Center members for metabolomics and proteomics. During the last funding period, three new mass spectrometers were added to the facility. These included two Bruker mass spectrometers (an Autoflex MALDI-TOF and an Esquire HCT ion trap) and a Waters/Micromass Quadrupole Time-of-flight (Q-TOF) mass spectrometer, specifically to enhance the study of proteins and lipids. Dr. Michael Thomas, a leading expert in the analysis of lipids and lipid metabolites by mass spectrometry, has joined the laboratory as a co-director with Dr. Mark Lively. The addition of Dr. Thomas, and his staff and instrumentation, has enhanced the ability of the BRL to meet the needs of the Cancer Center program members in the especially important areas of proteomics and metabolomics. Together, Drs. Lively and Thomas provide significant expertise in biochemistry of proteins and DNA, mass spectrometry, and analytical methods to enhance and promote cancer research in the CCCWFU.

生物分子资源实验室（BRL）是一个使用量很大的核心服务实验室， 支持41个癌症中心的研究实验室活动的综合癌症中心 2005财政年度。BRL为中心提供具有重要战略意义的分析服务 1）DMA、肽、蛋白质的表征和2）脂质的鉴定和定量， 蛋白质和肽。这些服务包括五种不同类型的质谱分析， 蛋白质、肽和脂质、自动DNA序列分析、RNA和DNA合成 寡核苷酸、肽和蛋白质的纯化以及肽的化学合成。关于BRL 在本报告所述期间分析了14 000多个样本。DNA序列分析（7924份样本） 和质谱分析（6403个样本）是BRL中使用最频繁的核心服务。的 蛋白质/肽和脱氧核糖核酸化学服务占另外956个样本。癌症实验室 2005财政年度，中心成员获得了BRL提供的49%的服务。 自上次审查以来，BRL已大幅扩大。质谱服务已添加到 满足癌症中心成员对代谢组学和蛋白质组学的重大需求。在过去 在供资期间，该设施增加了三台新的质谱仪。其中包括两个布鲁克质量 质谱仪（Autoflex MALDI-TOF和Esquire HCT离子阱）和沃茨/Micromass四极杆质谱仪（Micromass Quadrupole 飞行时间（Q-TOF）质谱仪，专门用于增强蛋白质和脂质的研究。博士 迈克尔托马斯，一个领先的专家在分析脂质和脂质代谢物的质谱， 与马克·莱弗利博士一起加入实验室担任联合主任。托马斯博士和他的工作人员的加入， 仪器，提高了BRL的能力，以满足癌症中心计划的需求 成员在蛋白质组学和代谢组学的特别重要的领域。莱弗利博士和 托马斯在蛋白质和DNA的生物化学、质谱和分析方面提供了重要的专业知识。 加强和促进CCCWFU癌症研究的方法。