LC/MS/MS Purchase

描述（由申请人提供）：科罗拉多大学医学院将在NCRR共享仪器资助计划的支持下购买LC/MS/MS仪器。为了满足日益增长的对多肽和蛋白质分离和鉴定的需求，需要该仪器。增加的需求是由于学院几个系的师资队伍扩大，蛋白质组学计划的扩大，以及生物分子结构新的跨系项目的建立。所要求的文书将大大改善现有资源，并将加速下列系统的研究：

DESCRIPTION (provided by applicant): The University of Colorado School of Medicine will purchase a LC/MS/MS instrument with support for the NCRR Shared Instrumentation Grant Program. This instrument is required to meet the growing demand for the separation and identification of peptides and proteins. The increased demand results from expansion of the faculty in several departments in the School, the expansion of proteomics initiatives, and the establishment of a new interdepartmental Program in Biomolecular Structure. The requested instrument will vastly improve the existing resources and will accelerate research in the following systems: Methods development for glycoproteomics; identification of cell surface proteins through selection and structural determination of their tryptic glycopeptides. (B. Bendiak) Structure and mechanism in coordinated gene regulation. (M. Churchill) Development of new methodology for multidimensional liquid chromatographic separation of peptides and proteins for proteomic initiatives. (R.S. Hodges) Studies of yeast transcription involving Pafl/RNA polymerase II complex and mitochondrial RNA polymerase. (J.A. Jaehning) Genome scanning and identification of scaffold associated signaling complexes. (G. Johnson) Monitoring the degradation/chemical modification of recombinant proteins important to the biopharmaceutical industry during processing and storage. (M. Manning) Identification of yeast Cdc7-Dbf4 substrates in DNA repair and mutagenesis. (R. Sclafani) Identification and characterization of proteins interacting with epidermal growth factor receptor during endocytosis in normal and cancer cells. (A. Sorkin) Design of peptide analogs/mimetic of the hormones bradykinin and substance P and cloning of the substance P receptor in rat brain. (J. M. Stewart)